脱氧雪腐镰刀菌烯醇及其乙酰化产物的培养提取及制备

doi:10.7506/spkx1002-6630-201503025

食品科学

脱氧雪腐镰刀菌烯醇及其乙酰化产物的培养提取及制备

徐华，吴弼东，郭磊，陈佳，林妮妮，谢剑炜

军事医学科学院毒物药物研究所，抗毒药物与毒理学国家重点实验室，北京 100850

出版日期:2015-02-15 发布日期:2015-02-10

A Convenient Method for Preparation and Purification of Deoxynivalenol and Acetyldeoxynivalenol from Fusarium graminearum Culture

XU Hua, WU Bidong, GUO Lei, CHEN Jia, LIN Nini, XIE Jianwei

State Key Laboratory of Toxicology and Medical Countermeasures, Institute of Pharmacology and Toxicology,
Academy of Military Medical Sciences, Beijing 100850, China

Online:2015-02-15 Published:2015-02-10

摘要/Abstract

摘要：

以禾谷镰刀菌Fusarium graminearum大型分生孢子定量接种大米培养物，制备毒素粗提液，采用硅胶柱一步洗脱分离、分段收集可同时获得脱氧雪腐镰刀菌烯醇（deoxynivalenol，DON）和乙酰化脱氧雪腐镰刀菌烯醇（acetyldeoxynivalenol，AcDON），采用一步结晶法即可分别制备获得毒素纯品，并经红外光谱法、液相色谱-质谱联用技术及核磁共振氢谱等分析确证，液相色谱分析表明两种毒素纯度均大于98%。该培养提取及分离制备方法改变了常规多步骤繁琐过程，提供了一种切实有效的、大量制备纯化DON及乙酰化DON毒素的简便方法。

关键词: 禾谷镰刀菌, 脱氧雪腐镰刀菌烯醇, 乙酰化脱氧雪腐镰刀菌烯醇, 培养, 制备

Abstract:

Fusarium graminearum macroconidia were quantitatively inoculated in rice culture, and the crude extracts of the
toxins were prepared and subjected to silica gel column chromatography. Deoxynivalenol (DON) and acetyldeoxynivalenol
(AcDON) isolates were monitored by thin-layer chromatography and the fractions rich in these toxins were pooled and
concentrated, respectively. Both fractions yielded a crystalline material through a simple crystallization step. The two
compounds were identified and confirmed by infrared spectroscopy, liquid chromatography-mass spectrometry and
1H NMR analysis in comparison to the reference standards or the published data. The liquid chromatography analysis
showed that the purity of the two toxins were both greater than 98%. This method developed here has overcome the disadvantages
of the conventional cumbersome multi-step procedure, and provided a simplified and efficient strategy suitable for large-scale
production and purification of the mycotoxin DON and its acetylated products AcDON from Fusarium graminearum rice cultures.

Key words: Fusarium graminearum, deoxynivalenol, acetyldeoxynivalenol, culture, preparation

中图分类号:

TS201.3

徐华，吴弼东，郭磊，陈佳，林妮妮，谢剑炜. 脱氧雪腐镰刀菌烯醇及其乙酰化产物的培养提取及制备[J]. 食品科学, doi: 10.7506/spkx1002-6630-201503025.

XU Hua, WU Bidong, GUO Lei, CHEN Jia, LIN Nini, XIE Jianwei. A Convenient Method for Preparation and Purification of Deoxynivalenol and Acetyldeoxynivalenol from Fusarium graminearum Culture[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201503025.

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脱氧雪腐镰刀菌烯醇及其乙酰化产物的培养提取及制备

A Convenient Method for Preparation and Purification of Deoxynivalenol and Acetyldeoxynivalenol from Fusarium graminearum Culture

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