食品科学

• 生物工程 • 上一篇    下一篇

富硒乳酸菌的筛选及鉴定

曾议霆,郭溪浪,周 康*,刘书亮,韩新锋   

  1. 四川农业大学食品学院,四川 雅安 625014
  • 出版日期:2015-02-15 发布日期:2015-02-10

Screening and Identification of Se-Enriching Lactic Acid Bacteria

ZENG Yiting, GUO Xilang, ZHOU Kang*, LIU Shuliang, HAN Xinfeng   

  1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
  • Online:2015-02-15 Published:2015-02-10

摘要:

对5 种15 株乳酸菌的生长曲线进行了测定,并对亚硒酸钠添加质量浓度、耐硒和富硒能力进行比较,筛选出了富硒优势乳酸菌,并进行了生理生化和分子鉴定。结果表明:适宜的加硒时间为培养后的第6小时,培养时间为24 h。通过对9 株菌株比较,确定了较适宜的亚硒酸钠添加质量浓度为6 μg/mL。最终筛选出BC-25为富硒优势菌株,富硒量425.79 μg/g,硒转化率达27.00%,经16S rDNA分析鉴定其为植物乳杆菌(Lactobacillus plantaru)。

关键词: 乳酸菌, 富硒, 筛选, 鉴定

Abstract:

A total of 15 lactic acid bacteria derived from 5 species were studied by comparing their growth curves and
abilities to tolerate and enrich Se. We also identified and characterized the selected strain by biochemical and molecular
biological methods. The results showed that the optimum time to add Se was at the end of 6-hour culture period after which
the culture was carried out for another 18 hours. The optimum sodium selenite concentration for Se enrichment was found to
be 6 μg/mL by comparing 9 selected strains. The strain BC-25 was eventually selected as the best Se-enriching strain, which
could enrich 425.79 μg/g of Se with a transformation rate of 27.00%. The 16S rDNA sequence results indicated that BC-25
is Lactobacillus plantarum.

Key words: lactic acid bacteria, Se enrichment, screening, identification

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