食品科学

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白术多糖对树突状细胞表型及功能成熟的影响

汲广全,陈仁琼,郑建仙   

  1. 1.华南理工大学轻工与食品学院,广东 广州 510641;2.中山大学孙逸仙纪念医院皮肤科,广东 广州 510120
  • 出版日期:2015-02-15 发布日期:2015-02-10

Effect of Polysaccharides from Atractylodes macrocephala Koidz on Phenotypic and Functional Maturation of Dendritic Cells

JI Guangquan, CHEN Renqiong, ZHENG Jianxian   

  1. 1. School of Light Industry and Food Science, South China University of Technology, Guangzhou 510641, China;
    2. Department of Dermatology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China
  • Online:2015-02-15 Published:2015-02-10

摘要:

目的:研究白术多糖对人外周血来源的树突状细胞(dendritic cells,DC)表型和功能的影响。方法:应用流式细胞术检测DC表面标志分子HLA-DR、CD86、CD83和CD80的表达及吞噬FITC-dextran的情况,酶联免疫吸附实验检测DC分泌白细胞介素-12(interleukin-12,IL-12)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)情况,Western blotting检测DC表面Toll样受体4(Toll-like receptor 4,TLR4)。结果:白术多糖能够促进TLR4的表达,促进DC表面分子HLA-DR、CD86、CD83和CD80的表达,并且呈剂量依赖性,同时还使DC吞噬能力下降;白术多糖能够促进DC分泌IL-12和TNF-α。此外,TLR4抗体可以减少IL-12和TNF-α的产生。结论:白术多糖可以促进人外周血来源的DC表型及功能的成熟。

关键词: 白术, 树突状细胞, Toll样受体4

Abstract:

Purpose: To study the effect of polysaccharides from Atractylodes macrocephala Koidz (AMP) on phenotypic
and functional maturation of dendritic cells (DCs). Methods: The expression of toll-like receptor 4 (TLR4) was detected by
Western blotting and the phagocytosis and phenotypic maturation of DCs was determined by flow cytometry. Furthermore,
the production levels of IL-12 and tumor necrosis factor-α (TNF-α) were measured by enzyme-linked immunosorbent assay
(ELISA) in DCs stimulated by different concentrations of AMP (25, 50, 100, and 200 μg/mL). Moreover, the role of TLR4
in regulating IL-12 and TNF-α production was examined. Results: The expression levels of the markers of maturation such
as HLA-DR, CD86, CD83 and CD80 were increased by AMP in a concentrate-dependent manner. Furthermore, AMP
increased the production of IL-12 and TNF-α, promoted the expression of TLR4, and reduced the phagocytosis of DCs.
Using TLR4 antibody, the presence of anti-TLR4 antibody markedly decreased IL-12 and TNF-α release. Conclusion: These
data suggest that TLR4 is involved in the maturation of DCs induced by AMP.

Key words: Atractylodes macrocephala Koidz, dendritic cells, Toll-like receptor 4 (TLR4)

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