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固定化乳酸菌制备生物传感器测定抗生素方法的建立

乔立新1,庞广昌2   

  1. 1. 天津商业大学
    2. 天津商业大学生物技术与食品科学学院
  • 收稿日期:2014-11-23 修回日期:2015-06-19 出版日期:2015-08-25 发布日期:2015-08-17
  • 通讯作者: 庞广昌 E-mail:pgc@tjcu.edu.cn
  • 基金资助:
    国家自然科学基金资助项目

The Method of The Biosensor Preparation for Detecting Antibiotics by Immobilizing Lactobacillus

QIAO Li-XinPang Guangchang2   

  • Received:2014-11-23 Revised:2015-06-19 Online:2015-08-25 Published:2015-08-17
  • Contact: Pang Guangchang E-mail:pgc@tjcu.edu.cn

摘要: 用海藻酸钠-淀粉凝胶作固定剂,将乳酸菌固定到两片核微孔膜中间制成“三明治”式传感膜,然后将其固定到玻碳电极上制成生物传感电极。通过电化学工作站检测3种抗生素分别在不同浓度下的响应电流,经计算结果表明:该传感器固定瑞士乳杆菌的最适量为0.05g,此时对青霉素、链霉素、四环素的最低检测限分别为1×10-10g/mL、1×10-9g/mL、1×10-9g/mL,检出时间为4min,明显优于国内外对抗生素残留量的要求。而且经测定表明该电极在37℃的MRS培养基中至少可以稳定保存7d,低温保藏应该可以保存更长时间,说明电极性能比较稳定。总之,固定化乳酸菌制备的生物传感器提供了一种新的定量测定抗生素的方法,其检测灵敏度高、成本低、简单、快速,不仅适用于抗生素残留的定量化快速检测,而且可以实现对多种类型的抗生素进行检测。

关键词: 瑞士乳杆菌, 生物传感器, 青霉素, 链霉素, 四环素

Abstract: Using sodium alginate-starch gel as a fixing agent, lactobacillus helveticus was fixed between two nuclear microporous membranes to make a sandwich-type sensing membrane, which was then fixed to a glassy carbon electrode to make a biosensor electrode. By electrochemical workstation tested the current respectively of the 3 kinds of antibiotics in different concentration response, the calculation results showed that: The sensor fixed the most appropriate quality for lactobacillus helveticus was 0.05g, and now the limits of detection concentration (LDC) of the sensor to penicillin、streptomycin、tetracycline, respectively were 1×10-10g/mL、1×10-9g/mL、1×10-9g/mL, the detection time were 4min, so it’s obviously better than the requirement for residual antibiotic at home and abroad. By the measurement it showed that the electrode in 37℃ MRS medium could keep stable for 7d at least, and low temperature preservation should be able to save for a longer time, explainning the electrode performance was stable. In total, the microbial sensor by immobilizing lactobacillus helveticus provides a new method for quantitative determination of antibiotics, which is high detection sensitivity、low cost、simple and fast, and it is not only applicable to quantified and rapid detect the residual antibiotics, but also can detect a variety of types of antibiotics.

Key words: lactobacillus helveticus, biosensor, penicillin, streptomycin, tetracycline

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