食品科学

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花椒精对卵巢切除大鼠脂质代谢的影响

游玉明1,2,任文瑾1,刘庆庆1,刘 雄1,*   

  1. 1.西南大学食品科学学院,重庆 400715;2.重庆文理学院林学与生命科学学院,重庆 402160
  • 出版日期:2015-03-15 发布日期:2015-03-17

Effect of Oleoresin from Zanthoxylum bungeanum on Lipid Metabolism in Ovariectomized Rats

YOU Yuming1,2, REN Wenjin1, LIU Qingqing1, LIU Xiong1,*   

  1. 1. College of Food Science, Southwest University, Chongqing 400715, China;
    2. College of Forestry and Life Science, Chongqing University of Arts and Sciences, Chongqing 402160, China
  • Online:2015-03-15 Published:2015-03-17

摘要:

目的:探讨花椒精对卵巢切除大鼠脂质代谢的影响。方法:将40 只雌性Sprague-Dawley(SD)大鼠随机分为5 组,其中1 组进行伪切除手术(Sham)作为对照组,另外4 组进行双侧卵巢切除手术(ovariectomy,OVX),恢复饲喂一周后,分为空白组和灌胃花椒精高(15 mg/kg)、中(10 mg/kg)、低(5 mg/kg)剂量组。灌胃4 周后解剖,检测大鼠血脂、肝脂及小肠和粪便中胆汁酸含量等指标。结果:灌胃花椒精可降低血清中甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low density lipoproteincholesterin,LDL-C)和非高密度脂蛋白胆固醇(non-high density lipoprotein cholesterol,non-HDL-C)的浓度(p<0.05),升高HDL-C的浓度;降低肝脏中脂肪、TC、TG的含量(P<0.05);增加大鼠排便量、促进小肠内容物和粪便中胆汁酸以及粪醇、胆固醇等中性固醇的排泄,且存在明显的剂量效应。结论:花椒精可改善卵巢切除SD大鼠因雌性激素缺乏而引起的高脂血症,可能与促进胆汁酸和中性固醇的排泄有关。

关键词: 花椒精, 卵巢切除大鼠, 血脂, 胆汁酸, 中性固醇

Abstract:

Objective: To investigate the effect of oleoresin from Zanthoxylum bungeanum on lipid metabolism in
ovariectomized rats. Methods: Forty female Sprague-Dawley rats were randomly divided into five groups; one group
was sham-operated as sham control, and the other four groups were subjected to ovariectomy. After one week of feeding
postsurgery, the rats from the sham control and ovariectomy groups were daily orally administered with 0, 15 (OVX-HD),
10 (OVX-MD), and 5 mg/kg (OVX-LD) Zanthoxylum bungeanum oleoresin. After four weeks, all the rats were sacrificed for
the analysis of triglyceride (TG), cholesterol (TC) in serum and liver, bile acids in small intestine and feces. Results: By the
administration of Zanthoxylum bungeanum oleoresin, the concentrations of triglyceride, cholesterol, low-density lipoprotein
cholesterol and non high-density lipoprotein cholesterol in serum were significantly decreased (P < 0.05) as well as the
concentrations of fat, triglyceride and cholesterol in liver, but the excretion of feces, bile acid contents in small intestine and
feces, and the excretion of neutral steroids were increased. Conclusion: Zanthoxylum bungeanum oleoresin can ameliorate lipid
metabolism in estrogen deficiency-induced hypercholesterolemia rats by promoting the excretion of bile acids and neutral steroids.

Key words: Zanthoxylum bungeanum oleoresin, ovariectomized rats, serum lipid, bile acids, neutral steroid

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