黄酒多糖对免疫缺陷小鼠血清免疫相关因子的影响

doi:10.7506/spkx1002-6630-201505030

食品科学

黄酒多糖对免疫缺陷小鼠血清免疫相关因子的影响

沈赤1,2，毛健1,2,3,*，陈永泉1，孟祥勇1,2,3，艾斯卡尔·艾拉提1,2,3

1.江南大学食品学院，江苏无锡 214122；2.江南大学粮食发酵工艺与技术国家工程实验室，江苏无锡 214122；
3.国家黄酒工程技术研究中心，浙江绍兴 312000

出版日期:2015-03-15 发布日期:2015-03-17

Effect of Polysaccharides from Chinese Rice Wine on Immunity-Related Cytokines in Immunodeficient Mice

SHEN Chi1,2, MAO Jian1,2,3,*, CHEN Yongquan1, MENG Xiangyong1,2,3, AISIKAER Ailati1,2,3

1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
2. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China;
3. National Engineering Research Center of Chinese Rice Wine, Shaoxing 312000, China

Online:2015-03-15 Published:2015-03-17

摘要/Abstract

摘要：

研究黄酒多糖对免疫缺陷小鼠血清免疫因子的影响。选择健康昆明小鼠，适应性喂养4 d后，分成5 组：空白组、环磷酰胺（cyclophosphamide，CY）模型组、CY＋黄酒多糖（低、中、高剂量）组，每组10 只。黄酒多糖按高、中、低3 个剂量（50、100、200 mg/（kg·d），均以体质量计）给小鼠灌胃，1 次/d，连续给药14 d，空白组和CY模型组给予生理盐水。第19天除空白组，其余4 组腹腔注射环磷酰胺，连续4 d。处死小鼠，眼眶取血，测定血清中白细胞介素-6（interleukin 6，IL-6）、干扰素-γ（interferon-γ，IFN-γ）、肿瘤坏死因子-α（tumor necrosisfactor-α，TNF-α）、免疫球蛋白（IgA、IgM、IgG）、补体（C3、C4）水平。结果表明，与CY组相比，黄酒多糖能提高IL-6、IFN-γ、TNF-α、免疫球蛋白（IgA、IgM、IgG）和补体（C3、C4）水平，并且随着低中高剂量效果依次增加。由此说明，黄酒多糖能提高免疫缺陷小鼠的免疫功能。

关键词: 黄酒多糖, 免疫缺陷小鼠, 环磷酰胺, 免疫活性

Abstract:

The objective of the present work was to study the effects of polysaccharides from Chinese rice wine on immunityrelated
cytokines in immunodeficient mice induced by cyclophosphamide (CY). After fifty mice were fed adaptively for
four days, they were divided into five groups: blank control, cyclophosphamide, cyclophosphamide + polysaccharides
from Chinese rice wine (50, 100 and 200 mg/(kg·d)). The mice in CRWP + CY (50, 100 and 200 mg/(kg·d)) groups were
administered with polysaccharides from Chinese rice wine by gavage once a day for 14 days, and the mice from the blank
control group and CY group were fed with the same volume of physical saline. Then, the mice in CY group and CRWP +
CY group were injected intraperitoneally with CY (100 mg/(kg·d)) once a day for 4 days. On the 22th day, the mice were
executed and the blood was collected from the retro-orbital plexus and centrifuged for analysis. Interleukin 6, interferon-γ
tumor necrosis factor-α, immunoglobulin A, immunoglobulin M, immunoglobulin G and complement C3, complement
C4 in mouse serum were measured by ELISA. Compared with the controls, interleukin 6, interferon-γ, tumor necrosis
factor-α, immunoglobulin A, immunoglobulin M, immunoglobulin G and complement C3, complement C4 in mouse serum
were increased. The level of interferon-γ in CRWP + CY (200 mg/(kg·d)) group was significantly higher than that in the
blank control group. These results indicate that polysaccharides from Chinese rice wine can enhance immune function in
immunodeficient mice especially at higher dose.

Key words: polysaccharides from Chinese rice wine, immunodeficient mice, cyclophosphamide, immunocompetence

中图分类号:

TS262.4

沈赤1,2，毛健1,2,3,*，陈永泉1，孟祥勇1,2,3，艾斯卡尔·艾拉提1,2,3. 黄酒多糖对免疫缺陷小鼠血清免疫相关因子的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201505030.

SHEN Chi1,2, MAO Jian1,2,3,*, CHEN Yongquan1, MENG Xiangyong1,2,3, AISIKAER Ailati1,2,3. Effect of Polysaccharides from Chinese Rice Wine on Immunity-Related Cytokines in Immunodeficient Mice[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201505030.

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黄酒多糖对免疫缺陷小鼠血清免疫相关因子的影响

Effect of Polysaccharides from Chinese Rice Wine on Immunity-Related Cytokines in Immunodeficient Mice

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