食品科学

• 营养卫生 • 上一篇    下一篇

荷叶提取物的镇静催眠作用

闫明珠1,2,钟 雨1,2,刘新民2,张泽生1,叶林虎2,曹方瑞2,肖冰心2,冯 利2,常 琪2,*   

  1. 1.天津科技大学食品工程与生物技术学院,天津 300457;
    2.中国医学科学院&北京协和医学院,药用植物研究所,北京 100193
  • 出版日期:2015-03-15 发布日期:2015-03-17

Sedative and Hypnotic Effects of the Extracts from Nelumbo nucifera Leaves

YAN Mingzhu1,2, ZHONG Yu1,2, LIU Xinmin2, ZHANG Zesheng1, YE Linhu2, CAO Fangrui2, XIAO Bingxin2, FENG Li2, CHANG Qi2,*   

  1. 1. School of Food Engineering and Biological Technology, Tianjin University of Science and Technology, Tianjin 300457, China;
    2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
  • Online:2015-03-15 Published:2015-03-17

摘要:

目的:研究荷叶提取物(乙醇提取物、生物碱、总黄酮和小分子水溶物)对小鼠的镇静催眠作用,筛选其活性组分。方法:利用空场实验、戊巴比妥钠阈下催眠剂量实验和延长戊巴比妥钠睡眠时间实验,给予小鼠灌胃荷叶50%乙醇提取物(150、450 mg/kg)和不同分离组分(30、90 mg/kg)后,观察其对小鼠的镇静催眠作用。结果:高剂量荷叶乙醇提取物和低、高剂量荷叶生物碱均能显著抑制小鼠的自主活动,增加阈下剂量戊巴比妥钠引起的小鼠入睡率,缩短戊巴比妥钠阈上剂量的小鼠入睡潜伏期,延长睡眠时间。结论:荷叶乙醇提取物具有显著的镇静催眠作用,生物碱是其主要活性成分。

关键词: 荷叶, 镇静, 催眠, 生物碱

Abstract:

Objective: The present study aimed to determine the sedative and hypnotic effects of 50% ethanol extract and
its different fractions prepared from Nelumbo nucifera leaves. Methods: Locomotion activity and pentobarbital-induced
sleeping tests were used to evaluate the sedative and hypnotic activities of 50% ethanol extract (150 and 450 mg/kg) and
different fractions (30 and 90 mg/kg) through gavage administration. Results: Ethanol extract (450 mg/kg) and alkaloid
fraction (30 and 90 mg/kg) significantly inhibited the locomotion activity of mice, increased the percentage of sleeping and
decreased the sleep latency and prolonged the sleeping time of mice. Conclusion: Nelumbo nucifera leaves possess sedative
and hypnotic effects and its alkaloids are the bioactive components for these activities.

Key words: Nelumbo nucifera leaves, sedative, hypnotic, alkaloids

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