食品科学

• 工艺技术 • 上一篇    下一篇

中心组合设计优化芋头浆糖化工艺

戴缘缘,潘丽军*,姜绍通,吴学凤,余振宇   

  1. 合肥工业大学农产品加工研究院,生物与食品工程学院,安徽省农产品精深加工重点实验室,安徽 合肥 230009
  • 出版日期:2015-03-25 发布日期:2015-03-17
  • 基金资助:

    安徽省科技专项资金项目(13Z03042)

Optimization of Saccharification Conditions of Taro Pulp by Central Composite Design

DAI Yuanyuan, PAN Lijun*, JIANG Shaotong, WU Xuefeng, YU Zhenyu   

  1. Key Laboratory for Agriculture Processing Product of Anhui Province, Institute of Food Science and Engineering,
    School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
  • Online:2015-03-25 Published:2015-03-17

摘要:

为提高芋头浆中还原糖含量,对蒸煮、去皮、打浆所得的芋头浆进行预处理及液化后,通过单因素试验和中心组合试验设计优化芋头浆糖化条件,并采用响应面分析法建立其二次回归模型。获得芋头浆最佳糖化条件为:糖化酶添加量170 U/g、酶解温度55 ℃、酶解时间125 min、pH 4.7。此条件下芋头浆糖化液葡萄糖当量值可达(21.37±0.06)%,该值在模型响应值的95%预测区间[21.25%,22.62%]范围内,表明所建立的回归方程具有较好的预测效果。研究结果可为后续高品质芋头清汁饮料及发酵饮品的制备提供参考依据。

关键词: 芋头浆, 糖化, 葡萄糖当量值, 中心组合设计

Abstract:

In order to increase the content of reducing sugar in taro pulp, one-factor-at-a-time experiments and central
composite design method were used to optimize the saccharification conditions of the pretreated and liquefied taro pulp.
A quadratic regression model was proposed by response surface analysis. The optimal saccharification conditions were
established as follows: glucoamylase dosage, 170 U/g; temperature, 55 ℃; hydrolysis time, 125 min; and pH, 4.7. Under
these conditions, the dextrose equivalent value of taro pulp reached (21.37 ± 0.06)%, which was in the 95% prediction
interval of the response value. It was suggested that the regression equation had a good prediction capability. Therefore, the
optimal process may be applied to the preparation of high-quality fermented drinks and clarified juice beverages from taro.

Key words: taro pulp, saccharification, dextrose equivalent value, central composite design

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