食品科学

• 包装贮运 • 上一篇    下一篇

低质量浓度T-2处理对马铃薯块茎苯丙烷代谢活性的诱导

赵 莹,薛华丽,毕 阳*,唐亚梅,沈科萍   

  1. 甘肃农业大学食品科学与工程学院,甘肃 兰州 730070
  • 出版日期:2015-03-25 发布日期:2015-03-17
  • 通讯作者: 毕 阳
  • 基金资助:

    国家高新技术研究发展计划(863计划)项目(2012AA101607);甘肃省自然科学基金项目(1308RJZA280)

T-2 Toxin at Low Concentration Activates Phenylpropanoid Pathway in Potato Tubers

ZHAO Ying, XUE Huali, BI Yang*, TANG Yamei, SHEN Keping   

  1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China
  • Online:2015-03-25 Published:2015-03-17
  • Contact: BI Yang

摘要:

由镰刀菌引起的干腐病是导致甘肃省马铃薯块茎贮藏期间的主要病害。以‘陇薯3号’马铃薯为试材,用1 μg/mL T-2处理马铃薯块茎切片,观察T-2处理对马铃薯块茎切片损伤接种硫色镰刀菌(Fusarium sulphureum)后,其对病斑直径的抑制效果,以及块茎切片苯丙烷代谢关键酶活性及产物积累的影响。结果表明,T-2处理有效降低了块茎切片损伤接种Fusarium sulphureum的病斑直径,提高了切片苯丙氨酸解氨酶和4-香豆酰-辅酶A连接酶的活性,促进了木质素、总酚、类黄酮及花青素的积累。由此表明,低质量浓度T-2处理可通过诱导马铃薯的苯丙烷代谢增强块茎对干腐病的抗性。

关键词: 马铃薯, T-2毒素, 诱导抗性

Abstract:

Dry rot, caused by Fusarium sulphureum spp., is one of the most important postharvest diseases of potato tubers
in Gansu province. Potato tuber slices (cv. Long Shu No.3) were treated with T-2 toxin at 1 μg/mL. The efficacy of T-2 toxin
treatment on the lesion diameter of potato tuber slices inoculated with F. sulphureum, the activities of key enzymes involved
in phenylpropanoid pathway and metabolites were investigated. The results showed that T-2 toxin treatment significantly
decreased lesion diameter of potato tuber slices inoculated with F. sulphureum, and enhanced the activities of phenylalanine
ammonia-lyase (PAL) and coumaric acid-coenzyme A ligase (4CL), and also improved the accumulation of lignin, total
phenolic compounds, flavonoids and anthocyanin. It is suggested that T-2 toxin treatment at lower concentration induces
resistance against dry rot in harvested potato tubers by activating phenylpropanoid pathway.

Key words: potato, T-2 toxin, induced resistance

中图分类号: