食品动物源沙门氏菌血清型及对β-内酰胺类耐药性调查

doi:10.7506/spkx1002-6630-201507019

摘要/Abstract

摘要：

为了解我国食品动物源沙门氏菌血清型分布和对β-内酰胺类药物耐药情况。采用玻片凝集法和微量肉汤稀释法，对710 株分离自食品动物源沙门氏菌进行了血清型鉴定和10 种β-内酰胺类药物的敏感性实验。结果表明：710 株沙门氏菌有15 种血清型，主要血清型为鸡白痢沙门氏菌（S. pullorum）、德尔卑沙门氏菌（S. derby）、吉韦沙门氏菌（S. give）和菲利斯河沙门氏菌（S. fyris）；菌株对10 种β-内酰胺类药物呈不同程度的耐药性（耐药率13.66%～58.03%），66.19%菌株对3 种及以上药物同时耐药，耐药谱型复杂；菌株分离年代与耐药率无明显相关性，但整体上菌株对该类药物耐药率随时间延长呈上升趋势；耐药现象广泛存在于各种血清型，其中鸡白痢沙门氏菌、肠炎沙门氏菌、沙拉姆沙门氏菌和默尔斯沙门氏菌耐药菌株所占比例高达100%。以上结果说明，我国食品动物源沙门氏菌血清型呈多样性，对β-内酰胺类药物耐药现象严重，临床应谨慎使用该类药物，各动物中非致病性血清型菌株耐药现象严重，值得关注。

关键词: 食品动物源, 沙门氏菌, 血清型, 耐药性

Abstract:

Objective: To understand the serotype distribution and drug resistance to β-lactam antibiotics in Salmonella
isolated from animal derived foods in China. Methods: The slide agglutination and broth microdilution methods were used to
detect the serotype and resistant phenotype in 710 Salmonella isolates. Results: A total of 15 serotypes were identified among
these Salmonella isolates. The most common serotypes were S. pullorum, S. derby, S. give and S. fyris. The 710 strains
showed different resistance levels to 10 β-lactams and the resistance rate ranged from 13.66% to 58.03%. Meanwhile, 66.19%
of the strains showed multidrug resistance, and the drug resistance profile was very complex. In addition, no significant
correlation between resistance rate and year was observed, but overall the resistant rates increased with the extension of time.
All Salmonella serotypes were tolerant to the antibiotics differently, especially S. pullorum, S. enteritidis, S. salamae and
S. moers 100% of which had antibiotic tolerance. Conclusion: The resistance to β-lactams was serious in Salmonella isolates
from different serotypes. β-Lactams must be prudently used in veterinary clinical medicine.

Key words: animal derived foods, Salmonella, serotype, drug resistance

中图分类号:

S852.53

冯彩峰，林居纯*，张飞，舒刚，赵玲，高彤彤. 食品动物源沙门氏菌血清型及对β-内酰胺类耐药性调查[J]. 食品科学, doi: 10.7506/spkx1002-6630-201507019.

FENG Caifeng, LIN Juchun*, ZHANG Fei, SHU Gang, ZHAO Ling, GAO Tongtong. Salmonella Serotypes from Foods of Animal Origin and Their Resistance to β-Lactam Antibiotics[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201507019.

[1]	申进玲，赵丽娜，韩伟，许镇坚，蒋原，杨捷琳，郭德华，薛峰. 上海进口水产品中副溶血性弧菌耐药性、毒力基因和遗传特征[J]. 食品科学, 2021, 42(8): 264-269.
[2]	张鹏飞，付雪婷，赵春花，刘心雨，张杰，张萌，寇明莹，葛武鹏，王新. 羊奶粉生产中金黄色葡萄球菌分离鉴定及其分子特性和耐药性检测[J]. 食品科学, 2021, 42(6): 291-297.
[3]	郎晨晓，张一敏，朱立贤，梁荣蓉，毛衍伟，杨啸吟，韩广星，罗欣，董鹏程. 牛肉低温贮藏环境中沙门氏菌诱导耐酸响应的存在程度及其产生机制[J]. 食品科学, 2021, 42(6): 68-74.
[4]	吴有雪，吴美娇，田亚晨，王政，郭亮，刘程，方水琴，刘箐. 沙门氏菌检测生物传感器研究进展[J]. 食品科学, 2021, 42(3): 339-345.
[5]	杨其乐，丁一峰，张宇，聂若男，李亚萌，王佳，王小红. 沙门氏菌噬菌体LPST144尾纤维gp38的序列分析及其结合活性[J]. 食品科学, 2021, 42(2): 66-73.
[6]	孙悦，刘佳伊，陈璐，杜宏，白凤翎，吕欣然，张德福，郭晓华，励建荣. 抗耐药性大肠杆菌乳酸菌的筛选及抑菌机制[J]. 食品科学, 2021, 42(2): 121-127.
[7]	李琳琼，洪静，张丽君，高瑀珑. 酸胁迫对鼠伤寒沙门氏菌抗酸性、细胞膜及膜蛋白的影响[J]. 食品科学, 2021, 42(15): 27-36.
[8]	王虎虎，何淑雯，胡海静，白云，邵良婷，徐幸莲. 沙门氏菌生物菌膜形成的分子调控研究进展[J]. 食品科学, 2021, 42(13): 259-264.
[9]	闫玉卿，张一敏，董鹏程，毛衍伟，梁荣蓉，朱立贤，罗欣. 次氯酸钠对德尔卑沙门氏菌生物被膜的抑制作用及机制[J]. 食品科学, 2021, 42(13): 1-9.
[10]	李琳琼，洪静，张爱静，王鹏杰，高瑀珑. 酸胁迫处理对鼠伤寒沙门氏菌抗酸性的影响[J]. 食品科学, 2021, 42(1): 33-40.
[11]	王虹懿，刘芳，孙芝兰，苏萌萌，诸永志，王道营，徐为民，彭景. Helveticin-M与绿原酸复配对大肠杆菌和肠炎沙门氏菌的抑菌效果及其机制[J]. 食品科学, 2020, 41(3): 68-74.
[12]	张鹏飞，张杰，刘心雨，付雪婷，张萌，许学斌，吴聪明，姬华，王新. 上海市食源性耐甲氧西林金黄色葡萄球菌的分子特征及耐药性[J]. 食品科学, 2020, 41(20): 285-291.
[13]	盛焕精，李怡谰，王泽维，牛沁雅，孟令缘，曹晨阳，李伟，廉鲁昕，杨保伟. IncI1和IncN质粒阳性沙门氏菌耐药及质粒接合转移特征[J]. 食品科学, 2020, 41(18): 77-84.
[14]	彭亚博，李晓婷，方婷，李长城，魏钊异，田裕心，陈沁雯，刘万宁. 鸡肉中沙门氏菌和背景菌群生长动力学模型[J]. 食品科学, 2019, 40(9): 7-15.
[15]	田牧雨，张一敏，董鹏程，毛衍伟，梁荣蓉，朱立贤，罗欣. 沙门氏菌和单增李斯特菌诱导性耐酸响应机制的研究进展[J]. 食品科学, 2019, 40(5): 316-322.

食品动物源沙门氏菌血清型及对β-内酰胺类耐药性调查

Salmonella Serotypes from Foods of Animal Origin and Their Resistance to β-Lactam Antibiotics

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价