食品科学

• 生物工程 • 上一篇    下一篇

乳酸乳球菌生产γ-氨基丁酸条件的优化

陈 佳,杨诗颖,柯崇榕,黄建忠*   

  1. 福建师范大学生命科学学院,工业微生物发酵技术国家地方联合工程研究中心,福建 福州 350117
  • 出版日期:2015-04-15 发布日期:2015-05-05

Optimization of Conditions for the Production of γ-Aminobutyric Acid by Lactococcus lactis

CHEN Jia, YANG Shiying, KE Chongrong, HUANG Jianzhong*   

  1. National-Local United Engineering Research Centre of Industrial Microorganism Fermentation Technology, College of Life Science,
    Fujian Normal University, Fuzhou 350117, China
  • Online:2015-04-15 Published:2015-05-05

摘要:

采用析因设计和中心组合试验设计对乳酸乳球菌FJNU-GA1304产γ-氨基丁酸(γ-aminobutyric acid,GABA)的条件进行优化。完全析因设计优化后的细胞转化条件为:pH 3.5、反应温度40 ℃、反应时间24 h,谷氨酸钠质量浓度20 g/L和湿菌体质量浓度25 g/L;在单因素试验的基础上,通过筛选设计确定谷氨酸钠、玉米浆粉和葡萄糖质量浓度为主效因子。采用三因素三水平的中心组合试验对主效因子的交互作用进行分析,结果表明:最佳的培养基组成为谷氨酸钠9.50 g/L、玉米浆粉12.50 g/L、葡萄糖5.74 g/L、酵母膏5.00 g/L、K2HPO4 1.20 g/L、MgSO4 0.60 g/L。在最佳转化条件和发酵培养基组合下,GABA产量最高达9.06 g/L,比优化前4.80 g/L提高了88.8%。

关键词: &gamma, -氨基丁酸, 乳酸乳球菌, 析因设计, 筛选设计, 中心组合试验设计

Abstract:

Factorial design and central composite design were applied to optimize the yield of γ-aminobutyric acid (GABA)
produced by cultured Lactococcus lactis cells from monosodium glutamate in a buffer solution. A full factorial design was used to
optimize the conversion conditions as 40 ℃, pH 3.5 and 24 h. Using single factor designs, sodium glutamate, corn steep powder
and glucose concentration were identified as major factors affecting GABA production. The interaction effect of the major factors
at three levels each was analyzed by central composite design. The results showed that the optimal medium for Lactococcus
lactis consisted of 9.5 g/L sodium glutamate, 5.74 g/L glucose, 12.50 g/L corn steep powder, 5.00 g/L yeast extract, 1.20 g/L
K2HPO4 and 0.60 g/L MgSO4. Under these conditions, the yield of GABA was 9.06 g/L, which was increased by 88.8%
when compared with that (4.80 g/L) before optimization.

Key words: γ-aminobutyric acid, Lactococcus lactis, factorial design, screening design, central composite design

中图分类号: