食品科学

• 成分分析 • 上一篇    下一篇

刺参水煮液糖蛋白组成成分分析

陈 宁,赵 君,李月惠,高荣春,衣 萌,汪 珊,杨静峰,*   

  1. 大连工业大学食品学院,国家海洋食品工程技术研究中心,辽宁 大连 116034
  • 出版日期:2015-04-25 发布日期:2015-04-28
  • 通讯作者: 辛丘岩
  • 基金资助:

    国家自然科学基金青年科学基金项目(31301430)

Chemical Composition of Glycoprotein from Boiling Water Extract of Sea Cucumber

CHEN Ning, ZHAO Jun, LI Yuehui, GAO Rongchun, YI Meng, WANG Shan, YANG Jingfeng, XIN Qiuyan*   

  1. National Engineering Research Center of Seafood, School of Food Science and Technology,
    Dalian Polytechnic University, Dalian 116034, China
  • Online:2015-04-25 Published:2015-04-28
  • Contact: XIN Qiuyan

摘要:

目的:刺参水煮液是刺参加工过程中的副产物,通过对刺参水煮液营养成分的分析有助于将这种海产珍品加工废弃物转化为高附加值产品。方法:刺参水煮液经醇沉、透析、冻干,得到刺参水煮液糖蛋白(glycoproteinin sea cucumber boiling water,GSCB)粗制品;采用液相色谱和SepharoseCL-6B凝胶层析柱确定其糖蛋白组成成分及分子质量;采用液相色谱-质谱对1-苯基-3-甲基-5-吡唑啉酮(1-phenyl-3-methyl-5-pyrazolone,PMP)衍生化的GSCB单糖组成进行分析;采用液相色谱法对其氨基酸组成进行分析。结果:GSCB主要含有2 种糖蛋白,分子质量分别为964.3 kD和2.5 kD。GSCB含有氨基葡萄糖、甘露糖、氨基半乳糖、葡萄糖、半乳糖、岩藻糖6 种单糖;含有18 种氨基酸,其中含有8 种人体必需氨基酸,占氨基酸总量的38.38%。此外,半必需氨基酸组氨酸的含量在18 种氨基酸中占比最高,占比达到13.21%。结论:GSCB中含有酸性黏多糖物质,并含有多种必需氨基酸和半必需氨基酸,是一种良好的营养糖蛋白物质。对其深入开发将有利于变废为宝,开发出活性良好的功能保健食品。

关键词: 刺参, 水煮液, 糖蛋白, 单糖组成, 氨基酸组成

Abstract:

Aim: To analyze the nutrient compositions of boiling water extract of sea cucumber, a by-product of the processing
of this valuable seafood. Methods: Glycoprotein from sea cucumber boiling water (GSCB) was prepared from by alcohol
precipitation, dialysis and lyophilization. The components and molecular weight of the crude glycoprotein were analyzed by
high performance liquid chromatography (HPLC) and Sepharose CL-6B column chromatography, respectively. GSCB was
hydrolyzed, derivatized with 1-phenyl-3-methyl-5-pyrazolone, and then analyzed for monosaccharide composition by LCMS.
The amino acid analysis was carried out by HPLC. Results: Two kinds of glycoprotein in GSCB, with molecular weights
of 964.3 and 2.5 kD, respectively, were identified. GSCB was composed of glucosamine, mannose, galactosamine, glucose,
galactose and fucose. GSCB contained 8 kinds of essential amino acids, which account for 38.38% of the total amino acids.
Besides, the content of semi-essential amino acid, histidine, accounted for 13.21% of the total amino acids, which was
the most abundant among the 18 kinds of amino acids. Conclusion: Boiling water extract of sea cucumber contains acid
mucopolysaccharides and a high amount of essential amino acids. It is a good nutritive material and can be developed into
functional and healthy foods.

Key words: sea cucumber, boiling water, glycoprotein, monosaccharide composition, amino acid composition

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