食品科学

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GC-O-MS法检测句容产区巨峰葡萄香气成分分析

于立志1,马永昆1,2,*,张 龙2,代春华1,于怀龙1,李俊芳1   

  1. 1.江苏大学食品与生物工程学院,江苏 镇江 212013;2.镇江市果圣源食品科技有限公司,江苏 镇江 212009
  • 出版日期:2015-04-25 发布日期:2015-04-28
  • 通讯作者: 马永昆
  • 基金资助:

    镇江市农业科技支撑项目(NY2012021);江苏高校优势学科建设工程项目(PAPD)

Analysis of Aroma Composition of Kyoto Grape from Jurong by GC-O-MS

YU Lizhi1, MA Yongkun1,2,*, ZHANG Long2, DAI Chunhua1, YU Huailong1, LI Junfang1   

  1. 1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;
    2. Zhenjiang Guoshengyuan Food Technology Co. Ltd., Zhenjiang 212009, China
  • Online:2015-04-25 Published:2015-04-28
  • Contact: MA Yongkun

摘要:

目的:对句容产区巨峰葡萄的挥发性香气成分进行分析。方法:采用固相微萃取技术富集巨峰葡萄浆果中的挥发性香气成分,采用气相色谱-嗅闻-质谱联用技术进行检测,香气成分经质谱标准谱库检索并结合保留指数及嗅闻结果进行定性分析,采用内标法进行定量分析。结果:在巨峰葡萄中共鉴定出50 种挥发性香气成分,占检出香气总量93.05%,并对各香气化合物进行分类分析,确定14 种巨峰葡萄香气的特征成分及香气轮廓。结论:与其他产区巨峰葡萄特征香气成分对比,产区因素可能是导致不同葡萄产区巨峰葡萄特征香气成分差异的主要原因之一。

关键词: 巨峰葡萄, 气相色谱-嗅闻-质谱, 香气分析

Abstract:

Objective: To analyze the volatile aroma components of Kyoto grape from a vineyard in Jurong, Jiangsu province,
China. Methods: The volatile aroma components in Kyoto grape were extracted by sold phase micro-extraction (SPME),
and then detected by gas chromatography-mass spectrometry coupled with olfactometry (GC-O-MS). The qualitative
analysis of volatile aroma components was conducted by retrieving mass spectrometry standard spectral library based on
retention index coupled with olfactometry results and the internal standard method was used to conduct quantitative analysis.
Results: A total of 50 kinds of volatile aroma components in Kyoho grape berries were identified and classified, which
accounted for 93.05% of the total amount of aroma detected. Aroma profile of ripe Kyoho grapes was established and a
total of 14 kinds of characteristic volatile aroma components were determined. Conclusions: Noticeable differences in the
characteristic aroma components of ripe Kyoto grape from Jurong exist compared with other producing regions.

Key words: Kyoho grape, gas chromatography-olfactometry-mass spectrometry (GC-O-MS), aroma analysis

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