食品科学

• 生物工程 • 上一篇    下一篇

植物乳杆菌表面性质及对Caco-2细胞的黏附

李 清1,2,刘小莉1,王 英1,董明盛2,周剑忠1,*   

  1. 1.江苏省农业科学院农产品加工研究所,江苏 南京 210014;2.南京农业大学食品科技学院,江苏 南京 210095
  • 出版日期:2015-05-15 发布日期:2015-05-11

Surface Properties and Adhesion to Caco-2 Cells of Lactobacillus planturam Strains

LI Qing1,2, LIU Xiaoli1, WANG Ying1, DONG Mingsheng2, ZHOU Jianzhong1,*   

  1. 1. Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
    2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2015-05-15 Published:2015-05-11

摘要:

通过对10 株不同来源的植物乳杆菌进行自聚集能力、表面疏水性以及体外黏附Caco-2细胞能力的测定,探究植物乳杆菌表面性质与黏附能力之间的关系,利用LiCl对植物乳杆菌进行处理,探究参与植物乳杆菌对细胞黏附过程的物质。结果表明:植物乳杆菌3-4对Caco-2细胞的黏附能力最强。所选的不同植物乳杆菌之间自聚集能力、表面疏水性以及对Caco-2细胞的黏附能力存在差异性;对细胞的黏附能力与表面疏水性存在着显著的相关性(P<0.05),因此,自聚集能力和疏水性可以作为筛选具有高黏附细胞能力的植物乳杆菌的参考指标。同时LiCl处理前后,植物乳杆菌自聚集能力和对细胞的黏附能力均有所下降,表明菌株表面蛋白类物质及其他大分子物质均参与自聚集和黏附过程。

关键词: 植物乳杆菌, 自聚集, 疏水性, 黏附

Abstract:

The autoaggregation ability, cell surface hydrophobicity and in vitro adhesion to Caco-2 cells of 10 Lactobacillus
planturam strains from different sources were assessed, and the relationship between surface properties and adhesion to
Caco-2 cells. The compounds associated with the adhesion process were investigated by using LiCl-treated L. planturam
strains. The results showed that L. planturam 3-4 showed the strongest adhesion to Caco-2 cells. Significant differences
in autoaggregation, hydrophobicity and adhesion to Caco-2 cells were observed among the 10 L. planturam strains.
Significant correlation existed between adhesion and cell surface hydrophobicity. Autoaggregation and
hydrophobicity could be used as reference indexes for the screening of L. planturam strains with high adhesion
ability. Meanwhile, when treated with LiCl, the autoaggregation and adhesion of some L. planturam strains were
decreased, indicating that surface-bound proteins and other macromolecules play an important role in the process
of autoaggregation and adhesion.

Key words: Lactobacilus planturam, autoaggregation, hydrophobicity, adhesion

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