食品科学

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正交试验优化灵芝三萜白蛋白纳米体制备工艺及其分子表征

黄 婷1,范远景1,*,孟 静1,张 帆1,耿保玉1,刘培志2,王明和2   

  1. 1.合肥工业大学生物与食品工程学院,安徽 合肥 230009;2.安徽刘郎食品有限公司,安徽 宣城 242000
  • 出版日期:2015-05-25 发布日期:2015-05-08
  • 通讯作者: 范远景

Optimization of the Preparation Procedure and Molecular Characterization of Ganoderma lucidum Triterpenoids-Bovine Serum Albumin Nanoparticles by Orthogonal Array Design

HUANG Ting1, FAN Yuanjing1,*, MENG Jing1, ZHANG Fan1, GENG Baoyu1, LIU Peizhi2, WANG Minghe2     

  1. 1.合肥工业大学生物与食品工程学院,安徽 合肥 230009;2.安徽刘郎食品有限公司,安徽 宣城 242000
  • Online:2015-05-25 Published:2015-05-08
  • Contact: FAN Yuanjing

摘要:

研究用牛血清白蛋白(bovine serum albumin,BSA)为载体包裹灵芝三萜制备纳米体的工艺与其分子表征;采用去溶剂化-化学交联法制备灵芝三萜白蛋白纳米体,以白蛋白纳米体包封率为主要评价指标,采用正交试验设计法优化灵芝三萜白蛋白纳米体的配方。结果表明:将BSA 50 mg溶于5 mL去离子水中,取灵芝三萜8 mg溶于25 mL无水乙醇中,水相以400 r/min持续搅拌的同时以1 mL/min的速率滴加灵芝三萜乙醇溶液,加入0.25%的戊二醛溶液125 μL,室温条件下固化12 h,减压浓缩除去乙醇,得到的灵芝三萜白蛋白纳米体粒径为(189±11.13)nm,多分散指数为0.227±0.016,Zeta电位为(-31.4±6.74)mV,包封率为(80.10±1.05)%,载药量为(11.36±0.13)%,纳米体溶液于4 ℃放置30 d,稳定性良好。

关键词: 灵芝三萜, 牛血清白蛋白, 纳米体, 稳定性

Abstract:

Purpose: To prepare and characterize Ganoderma lucidum triterpenoids-bovine serum albumin (BSA) nanoparticles.
Methods: Ganoderma lucidum triterpenoids-BSA nanoparticles were prepared by desolvation-chemical crosslinking
method and the preparation conditions were optimized with respect to entrapment efficiency by orthogonal array design.
Results: Nanoparticles with an average particle size of (189 ± 11.13) nm, a polydispersity index (PDI) of 0.227 ± 0.016,
a zeta potential of (−31.4 ± 6.74) mV, an entrapment efficiency of (80.10 ± 1.05)%, and a drug-loading efficiency of
(11.36 ± 0.13)% were obtained by dropwise addition of 8 mg of Ganoderma lucidum triterpenoids dissolved in 25 mL of
absolute ethanol to 50 mg/mL aqueous solution of BSA while constantly stirring the mixture at a speed of 400 r/min and
subsequent prompt addition of 125 μL of 0.25% glutaraldehyde. The nanoparticles were stable at 4 ℃ for 30 d.

Key words: Ganoderma lucidum triterpenoids, bovine serum albumin, nanoparticles, stability

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