食品科学

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灵芝功能成分酸奶营养品质与风味物质分析

李广富,陈 伟*,范路平,戚慧颖,支晓庆,刘苑媛   

  1. 山东农业大学食品科学与工程学院,山东 泰安 271018
  • 出版日期:2015-05-25 发布日期:2015-05-08
  • 通讯作者: 陈 伟

Nutritional Quality and Aroma Components in Yogurt Supplemented with Functional Components of Ganoderma lucidum

LI Guangfu, CHEN Wei*, FAN Luping, QI Huiying, ZHI Xiaoqing, LIU Yuanyuan   

  1. College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
  • Online:2015-05-25 Published:2015-05-08
  • Contact: CHEN Wei

摘要:

以灵芝酸、灵芝多糖和脱脂乳为原料,制备灵芝多糖酸奶和灵芝酸酸奶,采用固相微萃取-气相色谱-质谱(solid-phase micro extraction-gas chromatography-mass spectrometry,SPME-GC-MS)联用技术对不同灵芝功能成分添加的酸奶的挥发性风味物质进行分析。结果表明,与空白对照酸奶相比,灵芝多糖酸奶和灵芝酸酸奶乳酸活菌数分别增加3.1 倍和1.5 倍,灵芝多糖可显著促进嗜热链球菌、植物乳杆菌增殖生长。经SPME-GC-MS测定,空白对照酸奶中检测出24 种挥发性风味化合物,质量分数0.12%灵芝多糖酸奶中检测出37 种挥发性风味化合物,质量分数0.09%灵芝酸酸奶中检测出30 种挥发性风味化合物,3 种酸奶的风味特征为酮、醛、酸、醇、酯类等化合物综合作用结果。与空白对照酸奶相比,灵芝多糖酸奶中酮类、醛类、醇类相对含量降低14.15%、3.00%、10.35%,酯、烃类相对含量提高14.01%、10.42%;灵芝酸酸奶中酮、酸、醇类化合物相对含量分别降低4.78%、2.22%、8.93%,醛、酯类相对含量增加5.31%、10.61%。结果表明灵芝酸、灵芝多糖酸奶中风味物质种类增加,功能品质提高。

关键词: 灵芝多糖, 灵芝酸, 乳酸菌, 风味物质, 固相微萃取-气相色谱-质谱法

Abstract:

Solid-phase microextraction (SPME) combined with gas chromatography-mass spectrometry (GC-MS) was
employed to analyze the volatile flavor compounds of skim milk yoghurts fortified with ganoderic acid or Ganoderma
lucidum polysaccharide (GLP), which were designated as GLA yogurt and GLP yoghurt, respectively. Results showed that
compared with control yogurt, the viable cell counts of lactic acid bacteria in GLP yogurt and GLA yogurt were increased
by 3.1 and 1.5 times. GLP significantly promoted the multiplication and growth of Streptococcus thermophilus and
Lactobacillus plantarum. A total of 24 volatile flavor compounds were identified in control yogurt while 37 and 30 volatile
flavor compounds in yoghurts supplemented with 0.12% GLP and 0.09% GLA, respectively, mainly including ketones,
aldehydes, acids, alcohols, and esters. Compared with control yogurt, the contents of ketones, aldehydes and alcohols in
GLP yogurt were reduced by 14.15%, 3.00% and 10.35% whereas the contents of esters and hydrocarbons were increased
by 14.01% and 10.42%,respectively; the contents of ketones, acid and alcohol compounds in GLA yogurt were reduced by
4.78%, 2.22% and 8.93%, respectively, whereas the contents of aldehyde and ester were increased by 5.31% and 10.61%,
respectively. The current study showed that the number of volatile flavor compounds in yogurt was increased and the
functional quality was improved by the addition of either ganoderic acid or GLP.

Key words: Ganoderma lucidum polysaccharide, ganoderic acid, lactic acid bacteria, aroma, solid-phase micro extractiongas chromatography-mass spectrometry (SPME-GC-MS)

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