食品科学

• 安全检测 • 上一篇    下一篇

市售生鲜鸡肉5 种主要食源性致病菌污染检测及分布概率评估

蒲承君1,崔兴博1,杜兰兰1,雷 娅1,张宏宇1,刘 芳2,董庆利1,刘 箐1,*   

  1. 1.上海理工大学医疗器械与食品学院,上海 200093;
    2.甘肃出入境检验检疫局国际旅行卫生保健中心,甘肃 兰州 730020
  • 出版日期:2015-05-25 发布日期:2015-05-08
  • 通讯作者: 刘 箐
  • 基金资助:

    大学生创新创业训练计划项目(XJ2013231);上海市科委重点支撑项目(13430502400);
    国家质检总局科技项目(GSCIQ_2010IK220)

Detection of Contamination of Five Main Foodborne Pathogens and Distribution Probability Assessment in Commercial Raw Chicken

PU Chengjun1, CUI Xingbo1, DU Lanlan1, LEI Ya1, ZHANG Hongyu1, LIU Fang2, DONG Qingli1, LIU Qing1,*   

  1. 1. College of Food Science and Medical Instrument, University of Shanghai for Science and Technology, Shanghai 200093, China;
    2. International Travel Health Care Center of Gansu Entry and Exit Inspection and Quarantine Bureau, Lanzhou 730020, China
  • Online:2015-05-25 Published:2015-05-08
  • Contact: LIU Qing

摘要:

采用聚合酶链式反应技术对上海市52 个市场的208 份鸡腿、鸡爪、鸡翅、鸡胗、鸡胸肉中出血性大肠杆菌O157∶H7、沙门氏菌、志贺氏菌、单增李斯特菌、阪崎肠杆菌5 种食源性致病菌进行快速检测,以了解不同鸡肉食用部位各种致病菌的分布规律,并根据β概率分布确立最具代表性的部位作为评估靶点。结果显示:在208 份样品中,大肠杆菌O157∶H7和沙门氏菌未检出;阪崎肠杆菌在鸡翅中检出1 株;志贺氏菌检出2 株,分布于鸡翅、鸡胗;单增李斯特菌检出11 株,检出率达5.29%,且在鸡翅中检出率最高(4 株),鸡腿(3 株)、鸡爪(3 株)部位检出率相当,鸡胗检出率最低(1 株)。

关键词: 聚合酶链式反应, 致病菌, 生鲜鸡肉, &beta, 概率分布, 评估靶点

Abstract:

In order to understand the distribution patterns of pathogens in different parts of raw chicken, PCR was used to
detect Escherichia coli O157:H7, Salmonella, Shigella, Listeria monocytogenes and Enterobacter sakazakii that distribute on
drumstick, claw, wing, breast and gizzard in 208 samples collected from 52 local markets in Shanghai. Then we established
evaluation of the target point according to beta probability distribution. The results indicated that no Escherichia coli
O157:H7 or Salmonella were detected in all samples, 1 strain of Enterobacter sakazakii was detected in chicken wing,
2 strains of Shigella were detected in chicken wing and gizzard, and 11 strains of Listeria monocytogenes were detected with
a detection rate of 5.29%, including 4 in chicken wing, 3 in drumstick, 3 in claw and 1 in gizzard.

Key words: PCR, pathogens, raw chicken, beta probability distribution, target point evaluation

中图分类号: