食品科学

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辉光放电低温等离子体技术对微生物的杀菌动力学及杀菌机制

李兆杰1,杨丽君1,*,刘小菁2,王 静1,刘玉敏1,李春喜1   

  1. 1.威海出入境检验检疫局,山东 威海 264205;2.威海职业学院,山东 威海 264210
  • 出版日期:2015-06-15 发布日期:2015-06-07

Bactericidal Kinetics and Mechanisms of Low Temperature Glow Discharge Plasma

LI Zhaojie1, YANG Lijun1,*, LIU Xiaojing2, WANG Jing1, LIU Yumin1, LI Chunxi1   

  1. 1. Weihai Entry-Exit Inspection and Quarantine Bureau, Weihai 264205, China;
    2. Weihai Vocational College, Weihai 264210, China
  • Online:2015-06-15 Published:2015-06-07

摘要:

应用研制的辉光放电低温等离子体杀菌设备,以大肠杆菌、志贺氏菌、金黄色葡萄球菌、单核细胞增生李斯特氏菌、黄曲霉菌、白色念珠菌6 种微生物为实验菌株,探讨辉光放电低温等离子体杀菌技术对不同微生物的杀菌动力学;应用扫描电镜、透射电镜、DNA电泳、光谱分析等技术,探讨辉光放电低温等离子体的杀菌机制。结果表明:辉光放电低温等离子体杀菌技术对大肠杆菌、志贺氏菌、金黄色葡萄球菌、单核细胞增生李斯特氏菌、黄曲霉菌、白色念珠菌6 种微生物的有效杀灭时间分别为2、1.5、3、4、10、10 min。依据杀菌动力学曲线,可以将6 种微生物准确分为革兰氏阴性细菌、革兰氏阳性细菌和真菌3 类,这可能与微生物的细胞壁结构有关;辉光放电低温等离子体可能是通过带电的高能粒子对细菌的细胞壁造成破坏,进而杀死细菌。该研究为辉光放电低温等离子体技术广泛用于微生物杀菌提供了重要的方法依据。

关键词: 低温等离子体, 辉光放电, 杀菌, 动力学, 机制

Abstract:

Low temperature plasma glow discharge sterilizer was developed and used to study the bactericidal kinetics of six
microorganisms including Escherichia coli, Shigella sonnei, Staphylococcus aureus, Listeria monocytogenes, Aspergillus
flavus and Candida albicans. Moreover, the bactericidal mechanisms were studied by scanning electron microscopy,
transmission electron microscopy, DNA electrophoresis and spectroscopy analysis. It was shown that low temperature glow
discharge plasma could kill E. coli, S. sonnei, S. aureus, L. monocytogenes, A. flavus and C. albicansi effectively in 2, 1.5, 3,
4, 10 and 10 minutes, respectively. According to the bactericidal kinetic curves, the six microorganisms were classified into
three categories, namely gram-negative bacteria, gram-positive bacteria and fungi, which may be resulted from the different
structures of cell walls of microorganisms. Furthermore, the charged particles with high energy produced by low temperature
glow discharge plasma played a dominant role in plasma-induced bacterial spore inactivation by destroying bacterial cell
walls. This study may provide an important method basis for the application of low temperature glow discharge plasma in
sterilization of microorganisms.

Key words: low temperature plasma, glow discharge, sterilization, kinetics, mechanism

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