食品科学

• 生物工程 • 上一篇    下一篇

淀粉酶产生菌MSP13筛选及其产酶条件初步优化

马晓梅,赵 辉*   

  1. 黑龙江大学生命科学学院,农业微生物技术教育部工程研究中心,黑龙江 哈尔滨 150080
  • 出版日期:2015-06-15 发布日期:2015-06-07

Screening of Amylase-Producing Strain MSP13 and Optimization of Fermentation Conditions

MA Xiaomei, ZHAO Hui*   

  1. Agricultural Microbiology Engineering Research Center, Ministry of Education, College of Life Science, Heilongjiang University,
    Harbin 150080, China
  • Online:2015-06-15 Published:2015-06-07

摘要:

从优质白酒窖泥中分离筛选出13 株产淀粉酶的兼性厌氧细菌,通过测定α-淀粉酶、β-淀粉酶、糖化酶和脱支酶酶活力,最终筛选出一株生产4 种淀粉酶活力均相对较高的菌株,并对其进行形态观察,生理生化指标和16S rDNA鉴定,确定该菌株为解淀粉芽孢杆菌,并将其命名为MSP13。对菌株MSP13产酶条件进行初步优化,确定其产酶的较佳条件是:CaCl2质量浓度0.15 g/L、MgSO4·7H2O质量浓度0.3 g/L,pH 5.5和温度37 ℃。优化后的菌株MSP13生产4 种酶的酶活力平均提高了31.645%。

关键词: 淀粉酶, 窖泥, 鉴定, 筛选, 白酒

Abstract:

Totally 13 strains of facultative anaerobic bacteria that can produce amylase were isolated from a highquality
liquor pit mud. One strain was screened by determining the activities of α-amylase, β-amylase, glucoamylase and
debranching enzyme. Through morphological observation, physiological and biochemical tests and molecular biological
methods, the screened strain was identified as Bacillus amyloliquefaciens and named as MSP13. Furthermore, the culture
conditions for amylase production were optimized to be 0.15 g/L CaCl2, 0.3 g/L MgSO4·7H2O, pH 5.5 and 37 ℃. This strain
under the optimal conditions could improve the four enzyme activities by 31.645% on average.

Key words: amylase, liquor pit mud, identification, screening, liquor

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