食品科学

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正交试验优化超声波辅助同时提取桑叶总黄酮、单宁工艺

钟雨珅1,王历琼1,韩小丹1,黄先智2,丁晓雯1,*   

  1. 1.西南大学食品科学学院,重庆市农产品加工及贮藏重点实验室,重庆 400716;
    2.西南大学蚕学与生物系统研究所,重庆 400716
  • 出版日期:2015-06-25 发布日期:2015-06-12
  • 通讯作者: 丁晓雯
  • 基金资助:

    国家桑蚕产业技术体系蚕茧综合利用项目(CARS-22-ZJ0503)

Optimization of Simultaneous Ultrasonic-Assisted Extraction of Total Flavonoids and Tannins from Mulberry Leaves

ZHONG Yushen1, WANG Liqiong1, HAN Xiaodan1, HUANG Xianzhi2, DING Xiaowen1,*   

  1. 1. Key Laboratory of Processing and Storage of Agricultural Products of Chongqing, College of Food Science, Southwest University,
    Chongqing 400716, China; 2. Institute of Bombycology and Biology, Southwest University, Chongqing 400716, China
  • Online:2015-06-25 Published:2015-06-12
  • Contact: DING Xiaowen

摘要:

采用超声波辅助法同时提取桑叶中总黄酮、单宁并用分光光度法对其含量进行测定,为这两类物质快速分离测定提供参考。在单因素试验基础上用正交试验优化,得到超声波同时提取桑叶总黄酮、单宁的最佳条件为:乙醇体积分数75%、料液比1∶30(g/mL)、提取温度80 ℃、提取时间10 min、超声功率128 W。在此条件下测得桑叶单宁提取量为6.32 mg/g,总黄酮提取量为18.55 mg/g。结论:与传统方法相比,超声波辅助提取法具有提取时间短、提取温度低、提取率高、精确度高的优点,且同时提取总黄酮、单宁的效果较好,适合于桑叶中总黄酮、单宁分离测定的提取。

关键词: 超声波, 总黄酮, 单宁, 桑叶

Abstract:

Ultrasonic-assisted extraction was adopted to simultaneously extract total flavonoids and tannins from mulberry
leaves and these two classes of compounds were spectrophotometrically determined. Results showed that the best conditions
for simultaneous ultrasonic-assisted extraction of total flavonoids and tannins from mulberry leaves were as follows: ethanol
concentration, 75%; material to liquid ratio, 1:30; extraction temperature, 80 ℃; extraction time, 10 min; and ultrasonic
power, 128 W. The yields of tannins and total flavonoids were 6.32 mg/g and 18.55 mg/g, respectively under these optimized
conditions. Compared with the traditional extraction methods, UAE achieved a higher extraction efficiency and a higher
accuracy in a shorter time at a lower extraction temperature, making it an appropriate method to extract total flavonoids and
tannins from mulberry leaves.

Key words: ultrasonic, total flavonoids, tannins, mulberry leaves

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