食品科学

• 成分分析 • 上一篇    下一篇

贵州产地八角莲叶挥发性成分分析

李锦辉   

  1. 河南省对外科技交流中心,河南 郑州 450003
  • 出版日期:2015-06-25 发布日期:2015-06-12
  • 基金资助:

    河南省科技厅重点攻关项目(142102310147)

Analysis of Volatile Components in Leaves of Dysosma versipellis from Guizhou Province

LI Jinhui   

  1. Henan Science and Technology Exchange Center, Zhengzhou 450003, China
  • Online:2015-06-25 Published:2015-06-12

摘要:

分析贵州尧人山和茂兰保护区所产八角莲叶中挥发性成分并进行比较。采用顶空固相微萃取八角莲叶中的挥发性成分,气相色谱-质谱联用技术检测其成分,面积归一化法计算各成分的相对含量。结果显示,尧人山产八角莲叶中共分离39 种组分,鉴定出31 种成分,占色谱总流出峰面积的91.22%,其中桉油烯醇(22.34%)、β-萜品烯(20.59%)、柠檬烯(10.47%)、(1S)-α-蒎烯(6.56%)、(+)-喇叭烯(4.52%)、γ-依兰油烯(4.11%)和石竹烯(3.22%)等化合物含量较高;茂兰保护区产八角莲叶中共分离25 种组分,鉴定出23 种成分,占色谱总流出峰面积的87.77%,其中十二醛(15.58%)、桉油烯醇(13.63%)、p-伞花烃(6.95%)、γ-依兰油烯(4.89%)、癸醛(4.67%)、十一醛(4.14%)、d-杜松烯(3.97%)、α-荜澄茄油烯(3.91%)、β-桉叶油醇(3.40%)和γ-榄香烯(3.24%)等化合物含量较高。两者共有成分8 种,但其相对质量分数有较大差异。可见八角莲叶中挥发性成分的差异与其产地、生长环境密切相关。

关键词: 八角莲, 挥发性成分, 顶空固相微萃取, 气相色谱-质谱

Abstract:

This work presents a comparative analysis of the volatile components in the leaves of Dysosma versipellis from
Yaorenshan and Maolan nature reserve in Guizhou province of China. The volatile components were extracted from the
leaves of D. versipellis by headspace solid phase microextraction, identified by GC-MS and quantified by the peak area
normalization method. The results showed that 39 compounds were extracted from the leaves of D. versipellis grown in
Yaorenshan, among which 31 compounds were identified, together accounting for 91.22% of the total volatile components,
and the predominant components included espatulenol (22.34%), β-terpinene (20.59%), limonene (10.47%), (1S)-α-pinene
(6.56%), (+)-ledene (4.52%), γ-muurolene (4.11%) and caryophyllene (3.22%). A total of 25 compounds were extracted
from D. versipellis in Maolan reserve, among which, 23 were identified and their relative contents accounted for 87.77%
of the total volatile components. The most abundant volatile components included dodecanal (15.58%), espatulenol
(13.63%), p-cymene (6.95%), γ-muurolene (4.89%), decanal (4.67%), undecanal (4.14%), d-cadinene (3.97%), α-cubebene
(3.91%), β-eudesmol (3.40%) and γ-elemene (3.24%). Eight compounds were common to both habitats, but there were large
differences in their relative contents. The differences in volatile components of D. versipellis may be closely associated with
producing area and growing environment.

Key words: Dysosma versipellis (Hance) M. Cheng ex Ying, volatile constituents, HS-SPME, GC-MS

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