食品科学

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稳定性碳、氮、氢同位素在牦牛产地区分中的应用

项 洋,郝力壮,牛建章,张晓卫,柴沙驼*   

  1. 青海省高原放牧家畜营养与生态国家重点实验室培育基地,青海省高原放牧家畜动物营养与饲料科学重点实验室,
    青海高原牦牛研究中心,青海大学畜牧兽医科学院,青海 西宁 810016
  • 出版日期:2015-06-25 发布日期:2015-06-12
  • 通讯作者: 柴沙驼
  • 基金资助:

    国家重点基础研究发展计划(973计划)项目(2012CB722906);“十二五”国家科技支撑计划项目(2012BAD13B01);
    青海省重大科技平台建设项目(2011-Z-Y12A;2012-Z-Y08)

Stable Isotope Distribution of Carbon, Nitrogen and Hydrogen in Yak Meat from Different Regions

XIANG Yang, HAO Lizhuang, NIU Jianzhang, ZHANG Xiaowei, CHAI Shatuo*   

  1. State Key Laboratory of Cultivating Base of Plateau Grazing Animal Nutrition and Ecology of Qinghai Province,
    Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Qinghai Plateau Yak Research Center,
    Qinghai Academy of Animal Science and Veterinary Medicine, Xining 810016, China
  • Online:2015-06-25 Published:2015-06-12
  • Contact: CHAI Shatuo

摘要:

为确定稳定性同位素对牦牛肉溯源的可行性。利用同位素质谱仪测定来自青海省河南县、大通县,四川省红原县、九龙县的牦牛肉样品中粗脂肪的碳同位素及脱脂牦牛肉中的碳、氮、氢同位素。通过比较分析不同地域来源牦牛肉中稳定性碳、氮、氢同位素的组成特征。结果表明,各地区牦牛肉不同组织中的稳定性碳、氮、氢同位素存在显著差异(P<0.05)或极显著差异(P<0.01),脱脂牦牛肉中的稳定性碳同位素判别效果好于粗脂肪中稳定性碳同位素判别效果,同位素指标组合判别效果好于单一同位素指标的判别效果,稳定性氮、氢同位素指标组合分析是牦牛肉产地溯源的有效指标,准确率可达到85%。利用稳定性碳、氮、氢同位素指标与多元统计分析方法相结合可以对不同地域来源的牦牛肉进行有效的区分。

关键词: 牦牛肉, 溯源, 稳定性同位素, &delta, 13C, &delta, 15N, &delta, 2H

Abstract:

This study aimed to confirm the possible traceability of yak meat samples from different regions using stable
isotopes. The δ13C, δ15N, and δ2H values of yak meat samples (muscle crude fat and de-fatted muscle) from Henan county
and Datong county of Qinghai province, and Hongyuan county and Jiulong county of Sichuan province were tested using
isotope ratio mass spectrometry (IRMS). The composition characteristics of δ13C, δ15N, and δ2H of yak meat samples were
compared and analyzed. The results demonstrated that the δ13C, δ15N, and δ2H differed significantly among meat samples
from different yak tissues (P < 0.05 or P < 0.01). The distinguishing capacity for de-fatted muscle was better than for muscle
crude. When the indicators of stable isotope were taken into comprehensive consideration, yak meat samples from different
places was better distinguished as compared to the individual indicators, and the combined analysis of stable nitrogen and
hydrogen isotopes provided potential indicators for tracing the origin of yak meat samples with an accuracy up to 85%. It is
a promising approach to classify yak meat based on δ13C, δ15N and δ2H combined with multivariate statistical methods.

Key words: yak, traceability, stable isotope, δ13C, δ15N, δ2H

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