柘树果实的抗乳腺癌活性部分的提取、分离及结构鉴定

doi:10.7506/spkx1002-6630-201513010

食品科学

柘树果实的抗乳腺癌活性部分的提取、分离及结构鉴定

曹春廷1,2，孙丛龙2，白卫滨2,*，孙建霞3，李国强2，欧仕益2，杨勇1,*

1.四川农业大学食品学院，四川雅安 625014；2.暨南大学食品科学与工程系，广东广州 510632；3.广东工业大学轻工化工学院，广东广州 510006

出版日期:2015-07-15 发布日期:2015-07-08

Extraction, Separation and Structure Identification of Anti-breast Cancer Compounds from Cudrania tricuspidata Fruits

CAO Chunting1,2, SUN Conglong2, BAI Weibin2,*, SUN Jianxia3, LI Guoqiang2, OU Shiyi2, YANG Yong1,*

1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China;
2. Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China;
3. Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China

Online:2015-07-15 Published:2015-07-08

摘要/Abstract

摘要：

为了得到柘树果实中具有抗乳腺癌活性的化合物，以人乳腺癌细胞MDA-MB-231和人乳腺癌细胞MCF-7为细胞模型，通过噻唑蓝（methyl thiazolyl tetrazolium，MTT）比色法评价柘树果实石油醚浸膏、乙酸乙酯浸膏、以及正丁醇浸膏粗提取物部位的功能，实验发现乙酸乙酯部位具有明显的抑制乳腺癌细胞的活性，其对乳腺癌细胞MDA-MB-231和MCF-7的IC50分别为：75.4 μg/mL和66.8 μg/mL。采用Sephadex LH-20柱层析和硅胶柱层析对乙酸乙酯部位进行分离，采用单晶X衍射对得到的化合物进行结构鉴定,得到2 种晶体主成分，分别为4’-O-甲基-吡喃异黄酮、吡喃异黄酮。采用MTT方法对获得的2 种单体进行抗乳腺癌活性研究，发现吡喃异黄酮对乳腺癌细胞MCF-7的生长抑制率达到56.7%。结果表明，柘树果实乙酸乙酯部位含有抗乳腺癌成分，且分离得到2 种类异黄酮化合物，其中吡喃异黄酮体现出一定的抗乳腺癌活性。

关键词: 柘树果实, 分离提取, 抗乳腺癌, 结构鉴定

Abstract:

The purpose of the present work was to obtain anti-breast cancer compounds from Cudrania tricuspidata fruits. The
methanol extract of Cudrania tricuspidata fruits was sequentially extracted with petroleum ether (PE), ethyl acetate (AE) and n-butyl
alcohol (n-BuOH) to obtain PE, AE and n-BuOH fractions. MTT assay was used to evaluate the anti-breast cancer activity
of the methanol extract and its three fractions against breast cancer cell lines MDA-MB-231 and MCF-7. The results showed
that the AE fraction had significant inhibitory activity on MDA-MB-231 and MCF-7 cells with half maximal inhibitory
concentrations (IC50) of 75.4 and 66.8 μg/mL, respectively. Then, the AE fraction was further separated by silica gel
column chromatography and Sephadex LH-20 column chromatography assay. The structures of crystals obtained from this
separation process were identified using single-crystal X diffraction. Two major crystal components were identif??ied including
4’-O-methylalpinumisoflavone and alpinumisoflavone. As evaluated by MTT assay, the inhibitory rate of alpinumisoflavone
against MCF-7 cell growth was 56.7%. Therefore, the AE fraction from Cudrania tricuspidata fruits containing isoflavones
and alpinumisoflavones has potent anti-breast cancer activity depending on the presence of alpinumisoflavone.

Key words: Cudrania tricuspidata (Carr.) Bur. fruits, separation and extraction, anti-breast cancer, structural identification

中图分类号:

R151

曹春廷1,2，孙丛龙2，白卫滨2,*，孙建霞3，李国强2，欧仕益2，杨勇1,*. 柘树果实的抗乳腺癌活性部分的提取、分离及结构鉴定[J]. 食品科学, doi: 10.7506/spkx1002-6630-201513010.

CAO Chunting1,2, SUN Conglong2, BAI Weibin2,*, SUN Jianxia3, LI Guoqiang2, OU Shiyi2, YANG Yong1,*. Extraction, Separation and Structure Identification of Anti-breast Cancer Compounds from Cudrania tricuspidata Fruits[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201513010.

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柘树果实的抗乳腺癌活性部分的提取、分离及结构鉴定

Extraction, Separation and Structure Identification of Anti-breast Cancer Compounds from Cudrania tricuspidata Fruits

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