食品科学

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妊娠期大鼠补充苏氨酸锌对仔鼠金属硫蛋白基因MT1表达的影响

王光然,虞俊翔,姜雯雯,冯建萍,胡晓波,谢明勇*   

  1. 南昌大学 食品科学与技术国家重点实验室,江西 南昌 330047
  • 出版日期:2015-07-15 发布日期:2015-07-08

Effect of Oral Zinc Threoninate in Pregnant Rats on the Expression of Metallothionein Gene in Their Offspring

WANG Guangran, YU Junxiang, JIANG Wenwen, FENG Jianping, HU Xiaobo, XIE Mingyong*   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Online:2015-07-15 Published:2015-07-08

摘要:

为了探讨母体补充苏氨酸锌对子代生长性能、金属硫蛋白(metallothionein,MT1)mRNA表达的影响,实验以苏氨酸锌为受试物,将90 只受孕SD大鼠随机分成5 组,并于妊娠期第6~15天进行灌胃补充不同剂量苏氨酸锌。母鼠分娩后将仔鼠进行常规饲养至1 月龄,测量仔鼠的体质量、身长和尾长,将各组仔鼠断颈处死后取肝脏、肾脏、脾脏和胸骨等组织进行RNA的提取并测定MT1基因相对表达量。结果表明:与空白对照组相比,苏氨酸锌各剂量组仔鼠体质量有显著增加(P<0.05)。与溶媒对照组和空白对照组相比较,苏氨酸锌各剂量组仔鼠的MT1 mRNA表达量高,且差异显著(P<0.05),并且1 000 mg/kg苏氨酸锌处理组各组织中MT1的基因表达量要高于其他组。本结果表明:妊娠期补充苏氨酸锌能显著提高仔鼠MT1 mRNA表达水平,并能在一定程度上增加仔鼠体质量。

关键词: 苏氨酸锌, 金属硫蛋白mRNA, 相对表达量

Abstract:

To observe the effect of zinc threoninate (Thr-Zn) orally supplemented to pregnant rats on the growth
performance and metallothionein (MT) mRNA expression in their offspring, 90 pregnant SD rats were randomly divided
into 5 groups which were administered daily with Thr-Zn at different doses by gavage during days 6 to 15 of pregnancy.
After birthing, the newborn rats were routinely fed for a full month. After measuring the body weight, body length and
tail length, the liver, kidney, spleen, and sternum of the offspring rats from each group were harvested for extracting RNA
and determining the relative expression levels of MT1 gene. Compared with the blank control group, body weights of the
offspring of rats treated with Thr-Zn increased significantly (P < 0.05). Compared with the blank control and solvent control
groups, MT1 mRNA expression levels in the offspring of rats treated with Thr-Zn were significantly higher (P < 0.05). The
expression level of MT1 mRNA in offspring rats with maternal Thr-Zn treatment at a dose of 1 000 mg/kg was higher
than that in other groups. Therefore, maternal Thr-Zn supplementation can significantly improve the expression level
of MT1 mRNA and increase the body weights of offspring rats.

Key words: zinc threoninate, metallothionein mRNA, relative expression level

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