食品科学

• 工艺技术 • 上一篇    下一篇

响应面试验优化[Bmim]Cl-Na2SO4双水相萃取分离琉璃苣种子总黄酮工艺

张喜峰1,罗光宏2,*,张芬琴1,崔 玮1   

  1. 1.河西学院农业与生物技术学院,甘肃 张掖 734000;
    2.河西学院 凯源生物技术开发中心 甘肃省微藻工程技术研究中心,甘肃 张掖 734000
  • 出版日期:2015-07-25 发布日期:2015-07-15
  • 通讯作者: 罗光宏
  • 基金资助:

    甘肃省中小企业创新基金项目(1047GCCG001);甘肃省高等教育科研项目(2014A-109;2014B-086);
    河西学院校长基金项目(XZ2014-29)

Aqueous Two-Phase System Based on [Bmim]Cl-Na2SO4 for Isolation of Total Flavonoids from Borage Seed

ZHANG Xifeng1, LUO Guanghong2,*, ZHANG Fenqin1, CUI Wei1   

  1. 1. College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, China;
    2. Microalgae Engineering Research Center of Gansu Province, Kaiyuan Bio-Tech Development Center, Hexi University, Zhangye 734000, China
  • Online:2015-07-25 Published:2015-07-15
  • Contact: LUO Guanghong

摘要:

利用1-丁基-3-甲基咪唑氯([Bmim]Cl)和硫酸钠(Na2SO4)双水相体系萃取分离琉璃苣种子总黄酮,首先对[Bmim]Cl、Na2SO4、总黄酮粗提物质量分数、pH值、无水乙醇质量分数进行单因素试验,确定双水相体系组成为21% [Bmim]Cl-25% Na2SO4。然后利用Box-Behnken试验,对总黄酮粗提物质量分数、pH值、无水乙醇质量分数进行了优化,结果表明:粗提物质量分数11.9%、pH 5.1、无水乙醇质量分数1.6%条件下琉璃苣种子总黄酮萃取率可达96.91%,与预测值97.19%接近。说明本优化工艺具有可行性。

关键词: 琉璃苣, 离子液体, 双水相

Abstract:

In this study, the potential use of ionic liquid-based aqueous two-phase system (ILATPS) for the extraction of
total flavonoids from borage seed was evaluated and optimized. The effects of independent variables including [Bmim]Cl,
Na2SO4, crude flavonoids, alcohol and pH were evaluated by single factor analysis. The optimized ILATPS consisting of
21% [Bmim]Cl and 25% Na2SO4 was selected based on its higher upper phase partitioning coefficient and recovery (extraction
yield). By adding 11.9% of crude flavonoids and 1.6% of absolute alcohol to the ILATPS and adjusting the pH to 5.1, the
maximum yield of flavonoids (96.91%) was observed, which was in good agreement with the predicted value of 97.19%. In
conclusion, the optimized process has good reliability.

Key words: Borago officinalis L., ionic liquid, aqueous two-phase system

中图分类号: