食品科学

• 生物工程 • 上一篇    下一篇

一株费氏丙酸杆菌生长特性及其代谢物抑菌作用分析

郑丽雪1,2,王荆楚2,时慧佳2,曹 雪3,齐 斌1,*   

  1. 1.常熟理工学院生物与食品工程学院,江苏 常熟 215500;2.苏州市食品生物技术重点实验室,江苏 常熟 215500;3.吉林中粮生化有限公司,玉米深加工国家工程研究中心,吉林 长春 130033
  • 出版日期:2015-08-15 发布日期:2015-08-17

Growth Characteristics and Metabolite Bacteriostatic Action of Propionibacterium freudenreichii

ZHENG Lixue1,2, WANG Jingchu2, SHI Huijia2, CAO Xue3, QI Bin1,*   

  1. 1. College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China;
    2. Suzhou Key Laboratory of Food and Biotechnology, Changshu 215500, China;
    3. National Engineering Research Center of Corn Deep Processing, Jilin COFCO Bio-chemical Co. Ltd., Changchun 130033, China
  • Online:2015-08-15 Published:2015-08-17

摘要:

对一株费氏丙酸杆菌生长特性及其代谢物抑菌作用进行研究。结果表明:该菌株生长周期约为10 d,第1~3天为其对数生长期,第4~8天为菌体生长的稳定期,第9~10天为衰亡期。在生长过程中,菌体利用糖的速率很快,还原糖在发酵1 d内几乎被消耗殆尽,发酵液pH值在发酵的第1天下降到4.6左右,此后一直到第10天,pH值基本稳定在4.6左右。在同等条件下,该丙酸杆菌代谢物对大肠杆菌的抑制作用优于对金黄色葡萄球菌和酵母菌的抑制作用。对代谢产物的分析结果表明,发酵产物中抑菌物质除了丙酸之外,还存在其他物质,初步判定为细菌素。

关键词: 丙酸杆菌, 生长特性, 抑菌物质, 细菌素

Abstract:

The growth characteristics and metabolite bacteriostasis of Propionibacterium freudenreichii were studied. The
results showed: the strain’s growth cycle was approximately 10 days, involving logarithmic phase (1–3 d), stability phase
(4–8 d) and decline phase (9–10 d). In the process of growth, sugar was consumed quickly, and almost completely
depleted in the first day. The pH after 1 day of fermentation dropped to 4.6 and then remained at 4.6 during the remaining
fermentation period. Under the same conditions, the bacteriostatic effect of Propionibacterium freudenreichii metabolites on
E. coli was better than those of Staphylococcus aureus and yeast. Bacteriocins were dominant metabolites with antimicrobial
activity besides propionic acid.

Key words: Propionibacterium freudenreichii, growth characteristics, antimicrobial substances, bacteriocins

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