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海带多糖对人肝癌细胞Bel-7402增殖的抑制作用及其机制

姜艳霞1,纪朋艳1,朱文赫1,张 巍1,徐俊杰1,李 妍1,雷钧涛2   

  1. 1.吉林医药学院生物化学与分子生物学教研室,吉林 吉林 132013;
    2.吉林医药学院药剂学教研室,吉林 吉林 132013
  • 出版日期:2015-08-15 发布日期:2015-08-17

Inhibitory Effect and Mechanism of Laminarin on Proliferation of Human Hepatoma Carcinoma Bel-7402 Cells

JIANG Yanxia1, JI Pengyan1, ZHU Wenhe1, ZHANG Wei1, XU Junjie1, LI Yan1, LEI Juntao2   

  1. 1. Department of Biochemistry and Molecular Biology, Jilin Medical University, Jilin 132013, China;
    2. Department of Pharmacy, Jilin Medical University, Jilin 132013, China
  • Online:2015-08-15 Published:2015-08-17

摘要:

目的:探讨不同质量浓度的海带多糖对人肝癌细胞Bel-7402增殖的抑制作用。方法:分别以质量浓度为5、10、20、30 mg/mL的海带多糖处理Bel-7402细胞,48 h后观察海带多糖对细胞增殖的抑制情况,Annexin V-FITC试剂盒检测细胞凋亡状况,酶联免疫吸附(enzyme-linked immunosorbent assay,ELISA)法检测Caspase-3活性,Western blotting法检测细胞内Caspase-3和甲胎球蛋白(alpha-fetoprotein,AFP)表达量。结果:海带多糖对Bel-7402细胞的增殖有明显抑制作用,最佳抑制剂量为30 mg/mL;随着海带多糖质量浓度的升高,细胞凋亡率明显增加(P<0.05),海带多糖处理组Bel-7402细胞内的Caspase-3表达水平同正常对照组细胞相比呈上升趋势,差异具有统计学意义(P<0.05);AFP蛋白表达水平呈下降趋势。结论:海带多糖对人肝癌细胞Bel-7402增殖具有抑制作用,其机制可能是激活Caspase-3,引起细胞凋亡和AFP蛋白表达水平下降所致。

关键词: 海带多糖, 细胞凋亡, 甲胎球蛋白, Caspase-3

Abstract:

Objective: To examine the inhibitory effect of different concentrations of laminarin on proliferation of human
hepatoma carcinoma Bel-7402 cells. Methods: Bel-7402 cells were cultured with different concentrations of laminarin for
48 h. The inhibitory effect of laminarin on the proliferation of Bel-7402 cells was evaluated by MTT (methyl thiazolyl
tetrazolium) assay. Cell apoptosis was detected using Annexin V-FITC Kit. The activity of Caspase-3 was examined by
enzyme-linked immunosorbent assay (ELISA). The expression of Caspase-3 and alpha-fetoprotein (AFP) was detected
by Western blotting. Results: Laminarin inhibited the proliferation of Bel-7402 cells in a concentration-dependent manner
with an optimal concentration of 30 mg/mL. The increased concentration of laminarin could accelerate the apoptosis rate of
cells (P < 0. 05). Caspase-3 activity significantly increased in the treatment groups when compared with the control group
(P < 0.05). The expression of Caspase-3 increased significantly and AFP expression decreased significantly in the treatment
groups. Conclusion: Laminarin can inhibit the proliferation of Bel-7402 cells, and the mechanism may be associated with
cell apoptosis caused by the activation of Caspase-3, and reduced expression of AFP.

Key words: laminarin, cell apoptosis, alpha-fetoprotein (AFP), Caspase-3

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