食品科学

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SDE结合GC-MS分析王致和臭豆腐中的特征香气成分

孙洁雯,杨克玉,李燕敏,陈怡颖,刘玉平,张玉玉*   

  1. 食品营养与人类健康北京高精尖创新中心,北京市食品风味化学重点实验室,
    北京工商大学 食品质量与安全北京实验室,北京 100048
  • 出版日期:2015-08-25 发布日期:2015-08-17
  • 通讯作者: 张玉玉
  • 基金资助:

    国家自然科学基金青年科学基金项目(31401604);“十二五”国家科技支撑计划项目(2014BAD04B06)

Analysis of Characteristic Aroma Compounds in Wangzhihe Stinky Tofu by Simultaneous Distillation-Extraction and Gas Chromatography-Mass Spectrometry (SDE-GC-MS)

SUN Jiewen, YANG Keyu, LI Yanmin, CHEN Yiying, LIU Yuping, ZHANG Yuyu*   

  1. Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Key Laboratory of Flavor Chemistry,
    Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048, China
  • Online:2015-08-25 Published:2015-08-17
  • Contact: ZHANG Yuyu

摘要:

采用同时蒸馏萃取的方法对王致和臭豆腐的挥发性成分进行提取,经气相色谱-质谱联用仪分析,采用计算的保留指数和NIST 11谱库检索,确定所含挥发性化合物的种类,并用内标法确定其在王致和臭豆腐中的含量,根据挥发性成分的香气特征,结合香气活性值,确定王致和臭豆腐中的特征性香气成分。经同时蒸馏萃取法结合气相色谱-质谱联用分析鉴定出44 种挥发性成分,包括醇类6 种(10.43 μg/g)、酯类17 种(6.88 μg/g)、酚类1 种(0.26 μg/g)、醛酮类3 种(0.15 μg/g)、硫醚类5 种(1.01 μg/g)、杂环类6 种(2.67 μg/g)、其他化合物6 种(2.66 μg/g)。其中含量较高的挥发性成分有正丁醇(7.21 μg/g)、正丙醇(2.33 μg/g)、乙酸乙酯(1.71 μg/g)、丁酸丁酯(1.44 μg/g)、乙酸丁酯(1.13 μg/g)等。而吲哚(0.70 μg/g)、正丁醇(7.21 μg/g)、正丙醇(2.33 μg/g)、二甲基二硫(0.42 μg/g)、二甲基三硫(0.36 μg/g)、二甲基四硫(0.15 μg/g)等挥发性成分对北京王致和臭豆腐特征性香味的贡献较大。

关键词: 臭豆腐, 同时蒸馏萃取, 特征香气成分, 气相色谱-质谱联用, 香气活性值

Abstract:

The characteristic aroma components of Beijing Wangzhihe stinky tofu were extracted by simultaneous
distillation-extraction (SDE), analyzed by gas chromatography-mass spectrometry (GC-MS) and quantified by internal
standard method. Based on the calculated retention indices and RI qualitative analysis, 44 compounds were identified. These
compounds included 6 alcohols (10.43 μg/g), 17 esters (6.88 μg/g), 1 phenol (0.26 μg/g), 3 carbonyl compounds (0.15 μg/g),
5 sulfur-containing ethers (1.01 μg/g), 6 heterocyclic compounds (2.67 μg/g), and 6 other compounds (2.66 μg/g). The major
aroma components were 1-butanol (7.21 μg/g), 1-propanol (2.33 μg/g), ethyl acetate (1.71 μg/g), butyl butyrate (1.44 μg/g),
and butyl acetate (1.13 μg/g). Indole, 1-butanol, 1-propanol, dimethyl disulfide, dimethyl trisulfide and dimethyl tetrasulfide
made a greater contribution to odor characteristics of Wangzhihe stinky tofu.

Key words: stinky tofu, simultaneous distillation-extraction (SDE), characteristic aroma components, gas chromatography-mass spectrometry (GC-MS), odor active values (OAV)

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