关键词: 北沙参, 香豆素, 茉莉酸甲酯, 水杨酸, 超声波

Abstract: We studied the influence of Methyl Jasmonate、Salicylic Acid and Ultrasonic on the production of cumarin ( imperatorin， isoimperatorin and psoraleae) and growth of callus of Radix Glehniae. The results showed that SA and MeJA could cause the callus brown, while a certain time and strength of ultrasound had a positive effect on callus vitality. At the same time, the results showed that the production of Cumarin could be promoted by the three kinds of factors. When the concentration of salicylic acid reached 8mg / L, the content of imperatorin reached the Maximum， which was 3.8 times higher than contrast; but the psoralen had a little effect by SA, when the concentration of salicylic acid reached 16mg / L, the production reached maximum, which was 1.7 times higher than contrast; When the concentration of salicylic acid reached 28-30mg / L, The maximum value of the content of the isoimperatorin and total cumarin which was 2.33 times higher than contrast; when the concentration of MeJA reached 400 μmol / L, the production of psoralen and isoimperatorin reached maximum, which respectively was 6.09 and 5.53 times higher than contrast, and the cumarin of total which was 5.11 times higher than contrast; when the time of ultrasound was 30min, the production of imperatorin、isoimperatorin and total cumarin reached maximum，which was respectively 5.15 、4.2and 3.55 times higher than contrast; when ultrasonic treatment 10min, the production of psoralen reached maximum, which was 1.5 times higher than contrast. In summary, comparison of three kinds of elicitors of Radix Glehniae callus，which was induced in the content of coumarin was detected,compared with ultrasonic and SA, MeJA could effectively promote the production of coumarin of Radix Glehniae .

Key words: Radix Glehniae, Cumarin, Methyl Jasmonate, Salicylic Acid, Ultrasound