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基于涂膜法FTIR的食用油羰基值快速检测研究

李易文1,于修烛2   

  1. 1. 西北农林科技大学食品科学与工程学院
    2. 西北农林科技大学
  • 收稿日期:2015-07-16 修回日期:2016-02-24 出版日期:2016-04-25 发布日期:2016-04-13
  • 通讯作者: 于修烛 E-mail:xiuzhuyu1004@hotmail.com
  • 基金资助:
    国家级大学生创新训练项目

Rapid FTIR Determination of Carbonyl Value in Edible Oils Using Polyethylene Film

1,YU Xiuzhu   

  • Received:2015-07-16 Revised:2016-02-24 Online:2016-04-25 Published:2016-04-13
  • Contact: YU Xiuzhu E-mail:xiuzhuyu1004@hotmail.com

摘要: 为了建立食用油羰基值快速检测方法,以菜籽油为实验材料,茴香醛为羰基标准物配制系列羰基值(0~60 mmol/kg)油样。用涂膜法采集油样光谱,建立羰基值与特征吸收峰1 703 /1 570 cm-1处(1 703 cm-1处峰高两点基线均为1 570 cm-1,下同)吸光度的关系。然而,不同种类零羰基值的食用油在特征吸收峰1 703 cm-1处的吸收相不同而引起基底效应。因此,利用二维相关振动光谱技术寻找1 703 cm-1处的相关吸收峰,以校正食用油间的基底效应。结果表明:油样在1 703 cm-1处的吸光度与羰基值高度线性相关,利用相关吸收峰3 472 cm-1校正后的模型为y=0.0054 x + 0.0909;相关系数(R)为0.9919, 标准偏差(SD)为0.0139。模型预测值与国标法测定值高度线性相关,所得方程的斜率接近于1,R值均在0.99以上,两种方法测定结果十分接近。表明涂膜法FTIR的食用油羰基值检测是可行的。

关键词: 食用油, FTIR, 羰基值, 检测

Abstract: To develop a rapid detection of carbonyl value (CV) in edible oils, anisaldehydes were gravimetrically added to carbonyl-free oil, using rapeseed oils as experimental materials and anisaldehydes as carbonyl reference. A series of CV (0~60 mmol/kg) oil samples were determined using polyethylene (PE) film as a spectrum acquisition accessory. A linear relationship between CV and absorbance at the characteristic absorption peak (1 703 /1 570 cm-1 , peak at 1 703 cm-1 and two base points at 1 570 cm-1) was established. However, different types of edible oils exhibit subtrate-effect, which shows that 1 703 cm-1 has variability among their even carbonyl-free samples. To correct subtrate effect, peak absorption at 3 472 cm-1, which was evaluated by the 2-D correlation spectroscopy technique, and it was used as the relevant absorption peak to 1 703 cm-1. Subsequently, a liner relationship between both peak height were established. Results indicated that there was a high linear correlation between the carbonyl value and the absorbance at 1 703 cm-1. The corrected model is y=0.0054 x + 0.0909. The correlation coefficient (R) is 0.9919 and the standard deviation is 0.0139. Moreover, there was a good relationship between the values dated from national standard method and the corrected model, with the slope close to 1, correlation coefficient more than 0.99. Both determinations are close to each other, showing that the PE film-based FTIR method for determination CV in edible oils was workable.

Key words: Edible oils, FTIR, carbonyl value, determination

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