枇杷花总黄酮、总三萜的大孔树脂制备工艺

doi:10.7506/spkx1002-6630-201518010

食品科学

枇杷花总黄酮、总三萜的大孔树脂制备工艺

陈晶1，李琪2，黄春萍2，张晓峰2，徐艳2，丁然2，张宏2,3,*

1.四川师范大学化学与材料科学学院，四川成都 610068；2.四川师范大学生命科学学院，四川成都 610101；
3.四川师范大学植物资源应用与开发研究所，四川成都 610101

出版日期:2015-09-25 发布日期:2015-09-11
通讯作者: 张宏
基金资助:
四川省科技厅第三批应用技术研究与开发项目（2009SZ005）

Preparation of Flavonoids and Triterpenoids from Flowers of Eriobotrya japonica (Thunb.) Lindl by Macroporous Resins

CHEN Jing1, LI Qi2, HUANG Chunping2, ZHANG Xiaofeng2, XU Yan2, DING Ran2, ZHANG Hong2,3,*

1. College of Chemistry and Materials Science, Sichuan Normal University, Chengdu 610068, China;
2. College of Life Sciences, Sichuan Normal University, Chengdu 610101, China;
3. Institute of Application and Development of Plant Resources, Sichuan Normal University, Chengdu 610101, China

Online:2015-09-25 Published:2015-09-11
Contact: ZHANG Hong

摘要/Abstract

摘要：

以枇杷花中总黄酮、总三萜含量作为检测指标，考察D101、AB-8、聚酰胺3 种大孔树脂对枇杷花提取物中总黄酮、总三萜的分离性能，考察4 种不同干燥方法对枇杷花总黄酮、总三萜含量的影响。结果表明，大孔树脂AB-8作为最佳分离树脂，上样量2.1 mL、吸附时间4 h、洗脱速率2 BV/h、洗脱体积3 BV时，枇杷花洗脱液中总黄酮含量为74.82%，收率为76.56%，总三萜含量为15.57%，收率为46.48%；减压干燥作为最佳干燥方法，真空度0.07 MPa、温度45 ℃、干燥时间10 h时，纯化物中总黄酮含量为76.07%，收率为77.87%，总三萜含量为15.89%，收率为47.09%，含水量为4.07%。研究结果表明，此制备工艺可行，为枇杷花总黄酮、总三萜的开发利用提供理论依据。

关键词: 枇杷花, 总黄酮, 总三萜, 分离纯化, 干燥

Abstract:

The total flavonoids and triterpenoids of loquat flower extracts were purified with D101, AB-8 and polyamide
resins and dried by atmospheric drying, vacuum drying, freeze drying and spray drying, respectively. The effectiveness
of these methods was tested based on the content and yield of total flavonoids and triterpenoids. The results showed that
AB-8 was the best macroporous resin for the separation and purification of flavonoids and triterpenoids, and vacuum drying
was the best drying method. The optimal conditions for purification were determined as follows: sample volume, 2.1 mL;
elution flow rate, 2 BV/h; adsorption time, 4 h; and eluent volume, 3 BV. Under these conditions, the content and yield of
flavonoids were 74.82% and 76.56% and the content and yield of triterpenoids were 15.57% and 46.48%, respectively. The
optimal conditions for vacuum drying were obtained by 10 h drying at 45 ℃ with a vacuum degree of 0.07 MPa. Under the
optimized drying conditions, flavonoids content was 76.07% with a yield of 77.87%, triterpenoids content was 15.89% with
a yield of 47.09% and moisture content was 4.07%.

Key words: loquat flowers, flavonoids, triterpenoids, separation and purification, drying

中图分类号:

R284.2

陈晶，李琪，黄春萍，张晓峰，徐艳，丁然，张宏. 枇杷花总黄酮、总三萜的大孔树脂制备工艺[J]. 食品科学, doi: 10.7506/spkx1002-6630-201518010.

CHEN Jing, LI Qi, HUANG Chunping, ZHANG Xiaofeng, XU Yan, DING Ran, ZHANG Hong. Preparation of Flavonoids and Triterpenoids from Flowers of Eriobotrya japonica (Thunb.) Lindl by Macroporous Resins[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201518010.

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枇杷花总黄酮、总三萜的大孔树脂制备工艺

Preparation of Flavonoids and Triterpenoids from Flowers of Eriobotrya japonica (Thunb.) Lindl by Macroporous Resins

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