食品科学

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高效液相色谱法对蓝莓果实中8 个有机酸含量的测定

徐玉涛1,李珂珂1,王贺新2,陈丽荣3,弓晓杰1,*   

  1. 1.大连大学医学院,辽宁 大连 116622;2.大连大学现代化农业研究院,辽宁 大连 116622;
    3.大连大学环境与化学工程学院,辽宁 大连 116622
  • 出版日期:2015-09-25 发布日期:2015-09-11
  • 通讯作者: 弓晓杰
  • 基金资助:

    辽宁省科技厅科学技术计划项目(2013204001;2014204007)

Determination of Eight Organic Acids in Blueberries by HPLC

XU Yutao1, LI Keke1, WANG Hexin2, CHEN Lirong3, GONG Xiaojie1,*   

  1. 1. College of Medical, Dalian University, Dalian 116622, China; 2. Modernization of Agricultural Research Center, Dalian University,
    Dalian 116622, China; 3. College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, China
  • Online:2015-09-25 Published:2015-09-11
  • Contact: GONG Xiaojie

摘要:

建立同时测定蓝莓果实中8 个有机酸类成分含量的高效液相色谱法,并利用该方法测定26 个不同品种蓝莓果实中的有机酸含量。结果表明,所测品种中塞拉的有机酸含量最高,达25.135 26 mg/g鲜果,最低的为克瑞顿,仅含2.368 31 mg/g鲜果。该方法简便、快速、准确,具有良好的重复性和回收率,可作为蓝莓果实中有机酸含量的测定方法。

关键词: 蓝莓, 有机酸, 柠檬酸, 高效液相色谱, 含量测定

Abstract:

A high performance liquid chromatography (HPLC) method was established for the simultaneous determination of
8 organic acids in blueberry fruit extract. This method was then used to determine the contents of organic acids in blueberries
from 26 cultivars. Among the blueberry cultivars tested, the content of total organic acids (25.135 26 mg/g fresh fruit) was
highest in Sierra and lowest in Croaton (only 2.368 31 mg/g fresh fruit). This method is simple, reproducible, accurate,
reliable and useful for the analysis of organic acids in blueberries.

Key words: blueberry, organic acid, citric acid, high performance liquid chromatography (HPLC), quantification

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