关键词: 果汁, 亚胺硫磷, 表面等离子共振传感器, 酶联免疫

Abstract:

In the present study, a polyclonal antibody-based method for the quantification of phosmet residues in apple,
peach and orange juice was presented using a surface plasmon resonance (SPR) sensor. Samples were directly diluted
with PBS to eliminate the matrix effect. Good accuracy and precision were obtained with mean spiked recoveries between
84.88% and 104.69% and coefficients of variation less than 6.70%, and the limit of detection (LOD) of juice samples was
0.1 μg/ L . The SPR method allowed rapid and accurate detection of phosmet and the correlation coefficient (R2) between
the data obtained using the method and high-performance liquid chromatography (HPLC) was 0.992. The chip used in the
SPR method detected phosmet in 30 min, and could be repeatedly used 30 times within 3 days, reducing the cost of tests.
The SPR method was advantageous in the terms of detection limit, accuracy and sensitivity rather other time saving in
single-residue analysis compared with the enzyme-linked immunosorbent assay (ELISA) and was more suitable for highthroughput
detection of multi-residues.

Key words: juice, phosmet, surface plasmon resonance (SPR) sensor, enzyme-linked immunosorbent assay (ELISA)