食品科学

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草酸脱羧酶促进草酸钙结晶溶解的体外研究

贺俊斌1,龙 寒1,林日辉2,*,梁宇薇1,陈阳阳1,高 华1,黄奇良2   

  1. 1.广西民族大学海洋与生物技术学院,广西高校微生物与植物资源利用重点实验室,广西 南宁 530006;
    2.广西民族大学化学化工学院,化学与生物转化过程新技术广西高校重点实验室,广西 南宁 530006
  • 出版日期:2015-10-15 发布日期:2015-10-20

Oxalate Decarboxylase Promotes the in vitro Dissolution of Calcium Oxalate Crystals

HE Junbin1, LONG Han1, LIN Rihui2,*, LIANG Yuwei1, CHEN Yangyang1, GAO Hua1, HUANG Qiliang2   

  1. 1. Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530006, China; 2. Key Laboratory of New Techniques for Chemical and Biological Conversion Process, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China
  • Online:2015-10-15 Published:2015-10-20

摘要:

在体外实验条件下,考察了加酶量、反应pH值、反应温度及其产物甲酸根等因素对草酸脱羧酶催化草酸降解从而促进草酸钙结晶重新溶解过程的影响。结果表明,在10 mL反应体系中,添加4 U草酸脱羧酶,反应pH 3.0,反应温度为37 ℃,反应进行1 周(168 h),可使近5%的草酸钙结晶重新溶解;实验结果表明,草酸降解产物甲酸根对草酸脱羧酶促进草酸钙结晶溶解产生抑制作用。

关键词: 草酸脱羧酶, 草酸钙, 溶解

Abstract:

Crystallization of calcium oxalate in the urinary system is one of the important causes of urinary stones, and
enzymatic degradation of oxalic acid for preventing calcium oxalate stones has become a research hotspot. Under in
vitro experimental conditions, the factors that affect the re-dissolution of calcium oxalate crystals catalyzed by oxalate
decarboxylase were studied. The results indicated that when 4 U of oxalate decarboxylase was added to the 10-mL reaction
system which was subsequently allowed to react at pH 3.0 and 37 ℃ for a week, the re-dissolution rate of calcium oxalate
crystals was approximately 5%. The experimental results showed that the degradation product formate produced an
inhibitory effect on the re-dissolution of calcium oxalate crystals catalyzed by oxalate decarboxylase. This experiment
provides a theoretical reference for the study of enzyme preparation to control calcium oxalate stones.

Key words: oxalate decarboxylase, calcium oxalate, dissolution

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