鳕鱼骨活性钙的正交试验优化及其生物利用度分析

doi:10.7506/spkx1002-6630-201520003

食品科学

鳕鱼骨活性钙的正交试验优化及其生物利用度分析

王珊珊1,2，李八方1,*，周德庆2，侯虎

1.中国海洋大学食品科学与工程学院，山东青岛 266003；2.中国水产科学研究院黄海水产研究所，山东青岛 266071

出版日期:2015-10-25 发布日期:2015-10-20
通讯作者: 李八方
基金资助:
国家高技术研究发展计划（863计划）项目（2014AA093508）；国家自然科学基金面上项目（31471606）；
青岛市博士后研究人员应用研究项目（ZQ51201415031）

Optimization of Preparation Process for Active Calcium from Cod Bone by Orthogonal Array Experiments and Its Oral Bioavailability in Vivo

WANG Shanshan1,2, LI Bafang1,*, ZHOU Deqing2, HOU Hu1

1. College of Food Science and Technology, Ocean University of China, Qingdao 266003, China;
2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

Online:2015-10-25 Published:2015-10-20
Contact: LI Bafang

摘要/Abstract

摘要：

目的：以水产加工下脚料鳕鱼骨为原料，探讨鳕鱼骨活性钙的最佳制备条件，并对其生物利用度进行研究。方法：对鱼骨进行基本组成分析与组织学观察；采用正交试验确定鱼骨活性钙的最佳提取条件；采用低钙饮食建立大鼠缺钙模型，检测大鼠血清生化指标、骨钙含量及钙代谢指标，对鱼骨活性钙的生物利用度进行探讨。结果：鳕鱼骨中钙与磷含量分别达到207.8 mg/g和100.9 mg/g，其比值接近2∶1。扫描电镜观察发现鳕鱼骨表面具有坚固致密的扁平板状羟基磷灰石结晶。活性钙最佳提取条件为提取温度100 ℃、提取时间120 min、酸混合比例（柠檬酸-苹果酸体积比）1∶2、酸质量分数15%，在此条件下提取率达到96.15%。与空白对照组相比，活性钙各剂量组能显著提高大鼠骨钙含量。钙代谢实验测定结果显示，活性钙高剂量组钙吸收率显著高于相同钙水平的碳酸钙对照组。结论：鳕鱼骨可作为一种优良的天然钙源，鱼骨活性钙能够作为一种有效的钙营养强化剂加以开发。

关键词: 鳕鱼骨, 活性钙, 生物利用度, 钙营养强化剂, 高值化利用

Abstract:

Objective: To determine the optimum conditions for the preparation of active calcium from cod bone through
orthogonal array experiments and to investigate its bioavailability. Methods: Compositional analyses and histological
observation of cod bone were performed. The optimum parameters for preparng active calcium from cod bone were obtained
by orthogonal array experiments. The calcium deficiency model in rats induced by low-calcium diet was used to investigate
the calcium bioavailability by detecting the contents of serum calcium and bone calcium and the indicators of calcium
metabolism. Results: The contents of calcium and phosphorous in cod bone were 207.8 mg/g and 100.9 mg/g, respectively,
indicating a ratio of approximately 2:1 between the two elements. Tightly and firmly combined hydroxyapatite crystals
characterized by the flat tabular shape were found under scanning electron microscopy (SEM) observation. The optimum
acid extraction conditions for active calcium from cod bone were determined as follows: extraction temperature, 100 ℃;
extraction time, 120 min; ratio of citric to malic acid, 1:2; and acid concentration, 15% leading to a calcium yield of 96.15%.
Compared with the blank control group, the active calcium at all doses investigated exhibited significantly higher contents
of femur calcium. The results of calcium metabolism experiments suggested that the calcium absorption of high dose group
was significantly higher than that of calcium carbonate group. Conclusions: These results demonstrate that cod bone is a
good calcium resource and the active calcium extracted from cod bone possesses excellent bioavailability and could be used
as a natural calcium supplement.

Key words: cod bone, active calcium, biological availability, calcium supplement, high-value utilization

中图分类号:

R336

王珊珊，李八方，周德庆，侯虎. 鳕鱼骨活性钙的正交试验优化及其生物利用度分析[J]. 食品科学, doi: 10.7506/spkx1002-6630-201520003.

WANG Shanshan1,2, LI Bafang1,*, ZHOU Deqing2, HOU Hu1. Optimization of Preparation Process for Active Calcium from Cod Bone by Orthogonal Array Experiments and Its Oral Bioavailability in Vivo[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201520003.

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鳕鱼骨活性钙的正交试验优化及其生物利用度分析

Optimization of Preparation Process for Active Calcium from Cod Bone by Orthogonal Array Experiments and Its Oral Bioavailability in Vivo

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