食品科学

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γ-谷维素对脂多糖诱导巨噬细胞RAW264.7炎症因子表达的影响

刘远锦1,田媛媛1,刘 博1,李新华2,陈娅娅1,曾琳娜1,杨 涛1,林亲录1,罗非君1,*   

  1. 1.中南林业科技大学食品科学与工程学院,稻谷及副产物深加工国家工程实验室,湖南 长沙 410004;
    2.中南大学湘雅医院消化内科,湖南 长沙 410008
  • 出版日期:2015-10-15 发布日期:2015-10-20

Effect of γ-Oryzanol on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in Macrophages

LIU Yuanjin1, TIAN Yuanyuan1, LIU Bo1, LI Xinhua2, CHEN Yaya1, ZENG Linna1, YANG Tao1, LIN Qinlu1, LUO Feijun1,*   

  1. 1. National Engineering Laboratory for Deep Processing of Rice and Byproducts, College of Food Science and Engineering,Central South University of Forestry and Technology, Changsha 410004, China;2. Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha 410008, China
  • Online:2015-10-15 Published:2015-10-20

摘要:

利用细菌脂多糖(lipopolysaccharide,LPS)刺激巨噬细胞RAW264.7形成炎症模型,评价γ-谷维素对巨噬细胞炎症因子表达的影响。在LPS刺激的RAW264.7细胞培养基中,添加不同浓度的γ-谷维素,分析培养基中炎症因子白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)的含量,以及NO2-/NO3-含量,发现γ-谷维素能抑制炎症因子的分泌;利用实时荧光定量聚合酶链式反应(real-time quantitative polymerase chain reaction,qPCR)分析mRNA表达水平,确定γ-谷维素能抑制炎症因子的基因表达;采用Western blotting分析进一步确定γ-谷维素能抑制炎症因子的蛋白表达。综上所述,γ-谷维素能明显抑制炎症因子IL-1β、TNF-α、IL-6、NO等分泌和表达。

关键词: &gamma, -谷维素, 炎症, 炎症因子, 米糠

Abstract:

In this study, we used macrophage models of lipopolysaccharide (LPS)-stimulated inflammation to evaluate the
effect of γ-oryzanol on the expression of inflammatory factors. After exposing RAW264.7 cells to different concentrations
of γ-oryzanol and LPS, we analyzed the contents of IL-1β, TNF-α, IL-6 and NO2-/NO3- (NO) in the macrophage culture
medium and found that γ-oryzanol could inhibit the secretion of inflammatory factors. Real-time quantitative PCR was
employed to analyzed mRNA expression levels of IL-1β, TNF-α, IL-6 and iNOS, and it was found that inflammatory gene
transcriptions were inhibited by γ-oryzanol. Western blotting analysis further confirmed that γ-oryzanol could decrease the
expression of IL-1β, TNF-α, IL-6 and iNOS protein. Our data showed that γ-oryzanol can significantly inhibit the expression
and secretion of inflammatory factors such as IL-1β, TNF-α, IL-6 and NO.

Key words: γ-oryzanol, inflammation, inflammatory factor, rice bran

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