食品科学
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刘天囡，徐梦佳，胡 冰，费群勤，徐冬兰，曾晓雄*
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LIU Tiannan, XU Mengjia, HU Bing, FEI Qunqin, XU Donglan, ZENG Xiaoxiong*
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摘要：
以湖南茯砖茶为原料通过热水浸提、树脂吸附分离得到茯砖茶多酚类物质，并采用酶动力学和荧光猝灭分析方法探讨了茯砖茶多酚类物质对胰脂肪酶的抑制作用。结果表明：茯砖茶多酚类物质对胰脂肪酶有抑制作用，半抑制浓度（IC50）为0.81 mg/mL，抑制类型为可逆非竞争性抑制，其抑制常数Ki为2.56 mg/mL。荧光光谱分析表明茯砖茶多酚类物质对胰脂肪酶具有荧光猝灭效应，与茯砖茶多酚类物质的结合可能造成了酶结构的改变，从而降低了酶的催化活性。
关键词: 茯砖茶, 多酚, 胰脂肪酶, 抑制作用
Abstract:
Polyphenols from Fuzhuan brick tea were obtained through hot water extraction and resin adsorption-separation process, and their inhibitory effect on pancreatic lipase was investigated by using enzyme kinetic analysis and fluorescence quenching method. The results showed that the polyphenols from Fuzhuan brick tea had inhibitory activity on pancreatic lipase with half inhibitory concentration (IC50) of 0.81 mg/mL. The inhibition was a type of reversible non-competitive inhibition, and the constant of inhibition (Ki) was 2.56 mg/mL. The fluorescence quenching analysis showed that the polyphenols had quenching effect on pancreatic lipase, and they reduced the enzyme activity, possibly by changing its structure due to their bonding to pancreatic lipase.
Key words: Fuzhuan brick tea, polyphenol, pancreatic lipase, inhibition activity
中图分类号:
TS272
TS201.3
刘天囡，徐梦佳，胡 冰，费群勤，徐冬兰，曾晓雄. 茯砖茶多酚类物质对胰脂肪酶活性的抑制作用[J]. 食品科学, doi: 10.7506/spkx1002-6630-201521010.
LIU Tiannan, XU Mengjia, HU Bing, FEI Qunqin, XU Donglan, ZENG Xiaoxiong. Inhibitory Activity of Polyphenols from Fuzhuan Brick Tea on Pancreatic Lipase[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201521010.
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链接本文: https://www.spkx.net.cn/CN/10.7506/spkx1002-6630-201521010
https://www.spkx.net.cn/CN/Y2015/V36/I21/46
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