全球副溶血弧菌环境菌株的基于碱基序列和肽链序列的多位点序列分型

doi:10.7506/spkx1002-6630-201521019

食品科学

全球副溶血弧菌环境菌株的基于碱基序列和肽链序列的多位点序列分型

徐嘉良1，杜小莉2，卢昕2,*

1.北京工商大学食品学院，食品添加剂与配料北京高校工程研究中心，北京 100048；
2.中国疾病预防控制中心传染病预防控制所，传染病预防控制国家重点实验室，北京 102206

出版日期:2015-11-15 发布日期:2015-12-03

Multilocus Sequence Typing of Global Environmental Strains of Vibrio parahaemolyticus Based on Nucleotide and Peptide Sequences

XU Jialiang1, DU Xiaoli2, LU Xin2,*

1. Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China;
2. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Online:2015-11-15 Published:2015-12-03

摘要/Abstract

摘要：

为了了解来自全球的副溶血弧菌环境菌株的群组结构和克隆复合体构成，利用PubMLST公共数据的数据，筛选其中具有完整序列分型（sequence type，ST）及肽序列型（peptide sequence type，pST）的来自全球的副溶血弧菌环境菌株数据，进行亚群分析和克隆复合体分析，并分别构建了基于ST型和pST型的最小生成树。结果表明，从数据库中共筛选到具有ST型及pST型的来自全球的副溶血弧菌环境菌株数据886 条，共含有663 个ST型，以ST3型为最多，但也仅含27 条菌株信息。其中，有436 条菌株数据来自中国（包括大陆、香港和台湾），覆盖了410 个ST型，每个ST型含1～4 条菌株数据，这436 条菌株数据又可分为128 个pST型，其中pST1型为最多，达116 条菌株数据。利用eBURST软件对数据进行分析，共发现了73 个组，483 个单体。STRUCTURE软件分析显示来自全球的副溶血弧菌环境菌株的适宜亚群数为4，各亚群内的样品平均距离为0.981 7。综上结果表明，来自全球的副溶血弧菌环境菌株具有高度多态性，可更细分为4 个亚群，MLST分型时以ST3型为多，AA-MLST分型时以pST1型为主。

关键词: 副溶血弧菌, 基于碱基序列的多位点序列分型, 基于肽链的多位点序列分型, 克隆复合体

Abstract:

Objective: To understand the group structure and clonal complex of worldwide environmental Vibrio
parahaemolyticus strains. Methods: Worldwide environmental V. parahaemolyticus strains with complete sequence types (ST)
and peptide sequence types (pST) were screened from the PubMLST public database, the subgroup analysis and complex
analysis was conducted, and the minimum spanning tree was established based on ST and pST types. Results: From the
database, 886 items were screened for analyzing the ST and pST types of worldwide environmental V. parahaemolyticus
strains, which belonged to 663 ST types with ST3 type being the most dominant, but only containing information on
27 strains. Out of these, 436 items came from China (including mainland, Hong Kong and Taiwan), including 410 ST types,
each of which contained information on 1– 4 strains and these 436 strains could be divided into 128 pST types, with pST1
type being the most dominant type, containing information on 116 strains. The eBURST software was used to analyze the
data and 73 groups and 483 singletons were observed. Analysis with the software STRUCTURE showed that appropriate
subset number of global V. parahaemolyticus strains was 4, and the average distance between samples in each subgroup was
0.981 7. Conclusion: Worldwide environmental V. parahaemolyticus strains in Asia are characterized by high polymorphism
and can be subdivided into 4 subgroups. ST3 type is the most dominant strain when MLST typing is used while pST1 type is
the most dominant strain by AA-MLST typing.

Key words: Vibrio parahaemolyticus, multilocus sequence typing, peptide based multilocus sequence typing, clonal complex

中图分类号:

Q939.99

徐嘉良，杜小莉，卢昕. 全球副溶血弧菌环境菌株的基于碱基序列和肽链序列的多位点序列分型[J]. 食品科学, doi: 10.7506/spkx1002-6630-201521019.

XU Jialiang, DU Xiaoli, LU Xin. Multilocus Sequence Typing of Global Environmental Strains of Vibrio parahaemolyticus Based on Nucleotide and Peptide Sequences[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201521019.

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全球副溶血弧菌环境菌株的基于碱基序列和肽链序列的多位点序列分型

Multilocus Sequence Typing of Global Environmental Strains of Vibrio parahaemolyticus Based on Nucleotide and Peptide Sequences

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