甲状腺素T3对四氧嘧啶诱导糖尿病小鼠胰腺的保护作用

doi:10.7506/spkx1002-6630-201521039

摘要/Abstract

摘要：

目的：研究甲状腺素T3对四氧嘧啶（alloxan，ALX）诱导糖尿病小鼠胰腺损伤的预防和保护作用。方法：24 只雄性昆明小鼠随机分成4 组，分别给予一次性腹腔注射生理盐水、ALX、ALX和甲状腺素T3、甲状腺素T3，在注射后1、4、6、12、24 h各时间点测定小鼠血液活性氧（reactive oxygen species，ROS）水平，并在注射后28 d内定期测定小鼠血糖水平变化。28 d后，处死小鼠，取出胰腺进行形态学和生物化学指标分析：检测甲状腺素T3对小鼠胰腺超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力以及还原型谷胱甘肽（glutathione，GSH）和氧化型谷胱甘肽（oxidized glutathione，GSSG）含量的影响，分析甲状腺素T3对胰腺细胞抗氧化酶相关基因Nrf2、SOD、GSH-Px、CAT，细胞凋亡相关基因Caspase-3、Caspase-9、Bax、Bcl-2表达的影响。结果：经ALX诱导的小鼠血糖和血液ROS水平极显著升高（P＜0.01），血液胰岛素水平极显著下降（P＜0.01），血液和胰腺抗氧化酶的活力均极显著下降（P＜0.01）。此外，抗氧化酶相关基因以及细胞凋亡相关基因表达水平均出现了极显著变化（P＜0.01）。注射甲状腺素T3能够改善由ALX引起的小鼠血糖水平上升和胰岛素分泌不足，显著或极显著地缓解抗氧化酶活力下降及抗氧化相关基因表达下调（P＜0.05或P＜0.01），极显著抑制Caspase-3、Caspase-9、Bax等促凋亡基因表达的升高（P＜0.01），促进抗凋亡基因Bcl-2的表达。结论：甲状腺素T3能够改善ALX诱导的糖尿病小鼠机体氧化还原状态，调节胰腺细胞凋亡相关基因的表达水平，保护胰腺组织，避免胰腺出现β细胞凋亡的情况，从而维持和保护胰腺调节血糖水平的功能。

关键词: 甲状腺素T3, 四氧嘧啶, 糖尿病, 胰腺

Abstract:

Objective: The protective effects of thyroid hormone T3 on the dysfunction of pancreas in alloxan (ALX)-induced
diabetic mice were investigated in this study. Methods: Twenty-four Kunming mice were randomly divided into four groups,
including negative control group, ALX group, ALX plus T3 (ALX + T3) group, and T3 group, which were subjected to
intraperitoneal administration of normal saline, ALX, ALX plus T3, and T3, respectively. The levels of reactive oxygen
species (ROS) in blood were tested at 1, 4, 6, 12, and 24 h after injection. Blood glucose was tested every 3 days. Mice were
sacrificed at 28 days after injection. Whole pancreas and plasma were utilized for morphological and biochemical analyses.
The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), the contents of reduced
and oxidative glutathione (GSH and GSSG), the expression levels of antioxidant enzyme genes including Nrf2, SOD,
GSH-Px, and CAT, and the expression levels of the apoptosis-associated genes Caspase-3, Caspase-9, Bax, Bcl-2 were
examined in each group. Results: ALX induced a high level of blood glucose and ROS (P < 0.01), low level of insulin in plasma
(P < 0.01), and lower activities of antioxidant enzymes in plasma and pancreas (P < 0.01), as well as down-regulation of the
antioxidant enzyme genes and apoptosis-related genes (P < 0.01). T3 could ameliorate the enhancement of blood glucose and
defective insulin secretion. The redox imbalance caused by ALX, including the decrease of antioxidant enzyme activities and
gene expression levels, was counteracted by T3. Moreover, the up-regulated pro-apoptosis genes (Caspase-3, Caspase-9 and
Bax) and down-regulated anti-apoptosis Bcl-2 gene were counteracted by T3 remarkably (P < 0.01). Conclusion: T3 could
improve the redox status of ALX-induced diabetic mice and protect pancreatic β-cells from cell death to maintain normal
physiological function of pancreas.

Key words: thyroid hormone T3, alloxan, diabetes, pancreas

中图分类号:

TS201.4

成向荣，丁寅翼，武旭，夏淑芳，施用晖，乐国伟. 甲状腺素T3对四氧嘧啶诱导糖尿病小鼠胰腺的保护作用[J]. 食品科学, doi: 10.7506/spkx1002-6630-201521039.

CHENG Xiangrong, DING Yinyi, WU Xu, XIA Shufang, SHI Yonghui, LE Guowei. Thyroid Hormone T3 Counteracts Pancreastic Dysfunction in Alloxan-Induced Diabetic Mice[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201521039.

[1]	牛博，庞广昌，鲁丁强. 花椒麻素的生物功能研究进展[J]. 食品科学, 2021, 42(9): 248-253.
[2]	陆敏涛，任廷远，杨建，陆龙发，秦礼康. 花椒精油对链脲佐菌素诱导的糖尿病小鼠糖代谢的影响[J]. 食品科学, 2021, 42(9): 115-122.
[3]	李阳，苏艳瑜，李国豪，李琪，王宇翔，丁玉松. 槲皮素通过Nrf2通路对糖尿病大鼠胰腺氧化损伤的拮抗作用机制[J]. 食品科学, 2021, 42(5): 208-214.
[4]	马永强，韩烨，张凯，王鑫，王峙力. 甜玉米芯多糖对胰岛素抵抗HepG2细胞糖代谢功能的影响[J]. 食品科学, 2021, 42(17): 170-176.
[5]	沈蒙，王维浩，康丽君，葛云飞，康子悦，肖金玲，全志刚，王娟，刘德志，赵姝婷，王金满，曹龙奎. 黑豆皮可溶性膳食纤维对糖尿病小鼠抗炎因子的调节作用[J]. 食品科学, 2020, 41(9): 81-85.
[6]	刘宸，冯鑫欢，豆智华，安淑静，刘梦娇，侯志梅，杨洁. 新疆双峰驼乳对链脲佐菌素诱导的糖尿病小鼠肝脏的保护作用[J]. 食品科学, 2020, 41(9): 74-80.
[7]	罗婷玉，杨玉辉，许云聪，高秋丽，施用晖，吴国卿，乐国伟. 蛋氨酸限制对高脂饮食诱导的肥胖小鼠胰岛功能损伤的改善作用[J]. 食品科学, 2020, 41(3): 111-119.
[8]	吴尚仪，李乳姝，石佳鑫，李兰馨，曹雪妍，杨梅，陶冬冰，岳喜庆. 乳清蛋白及其生物活性肽调节血糖功能研究进展[J]. 食品科学, 2020, 41(3): 266-271.
[9]	牛佳卉，付梦琪，周茜，王亚旭，吴梦颖，赵文，王颉. 亚麻籽油对链脲佐菌素诱导大鼠糖尿病肾病的保护作用[J]. 食品科学, 2020, 41(3): 120-126.
[10]	王云威，王景雪. 铁皮石斛多糖对2型糖尿病小鼠降糖降脂的作用[J]. 食品科学, 2020, 41(21): 127-132.
[11]	闫国森，郑环宇，孙美馨，丁阳月，张志华，许慧，陈昊. 抗性淀粉生理功能及作用机制的研究进展[J]. 食品科学, 2020, 41(21): 330-337.
[12]	刘芷君，林玲，雷郑延，曾文治，程福建，林全女，杨江帆. 茶籽皂苷对链脲佐菌素诱导的糖尿病大鼠血糖的影响[J]. 食品科学, 2020, 41(19): 179-184.
[13]	马永强, 张凯, 王鑫, 路雪纯, 林枞雨. 甜玉米芯多糖对糖尿病大鼠的降血糖作用[J]. 食品科学, 2020, 41(13): 169-173.
[14]	刘丹，邓建军，张超，张敬华，杨海霞. 原花青素防治II型糖尿病的研究进展[J]. 食品科学, 2019, 40(9): 302-308.
[15]	李露，张贤益，汤小芳，吴文英，李文娟. 膳食中碳水化合物与代谢综合征的研究进展[J]. 食品科学, 2019, 40(7): 268-273.

甲状腺素T3对四氧嘧啶诱导糖尿病小鼠胰腺的保护作用

Thyroid Hormone T3 Counteracts Pancreastic Dysfunction in Alloxan-Induced Diabetic Mice

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价