分散液液微萃取-高效液相色谱法测定枸杞中α-生育酚

doi:10.7506/spkx1002-6630-201522022

食品科学

分散液液微萃取-高效液相色谱法测定枸杞中α-生育酚

曹江平1，刘世巍1，解启龙2，易宗慧1，周继梅1，张玉龙1

1.宁夏师范学院化学化工学院，宁夏固原 756000；
2.中国科学院山西煤炭化学研究所，煤转化国家重点实验室，山西太原 030001

出版日期:2015-11-25 发布日期:2015-12-03
基金资助:
宁夏自然科学基金资助项目( NZ14275；NZ13216）；宁夏师范学院科研项目（YB201433）

Determination of α-Tocopherol in Goji Berries (Lycium barbarum) Using Dispersive Liquid-Liquid Microextraction Combined with High Performance Liquid Chromatography

CAO Jiangping1, LIU Shiwei 1, XIE Qilong2, YI Zonghui1, ZHOU Jimei1, ZHANG Yulong1

1. College of Chemistry and Chemical Engineering, Ningxia Normal University, Guyuan 756000, China;
2. State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001, China

Online:2015-11-25 Published:2015-12-03

摘要/Abstract

摘要：

建立基于分散液液微萃取样品前处理法，并结合高效液相色谱紫外检测技术测定枸杞中α-生育酚的含量，考察影响分散液液微萃取的因素包括萃取剂和分散剂类型及用量、pH值、离子强度、萃取时间和萃取温度。方法的线性范围为100～50 000 μg/L，r=0.999 7。在最优条件下，日内和日间重复性分别为2.9%和6.5%，检出限为3.1 μg/L，定量限为10.2 μg/L。并将该法成功应用于3 种市售枸杞中α-生育酚含量的测定，加标回收率为85.7%～106.3%，相对标准偏差为0.68%～4.62%。方法具有有机溶剂用量少、操作简单快捷、准确度和灵敏度高、重复性好等优点。

关键词: 分散液液微萃取, 枸杞, &alpha, -生育酚

Abstract:

A novel method for the determination of α-tocopherol in Goji berries (Lycium barbarum) was developed using
dispersive liquid-liquid microextraction (DLLME) coupled with high performance liquid chromatography by UV detection
(HPLC-UV). The influence of DLLME experimental parameters including type and volume of extraction solvents and
dispersers, sample solution pH, ionic strength, extraction time and temperature on extraction efficiency were examined.
The method offered excellent linearity over a range of 100–50 000 μg/L with correlation coefficient (r) of 0.999 7. Under
the optimum conditions, intra-day and inter-day repeatability values, expressed as relative standard deviation (RSD), were
2.9% and 6.5%, respectively. The limit of detection (LOD) and limit of quantitation (LOQ) were 3.1 μg/L and 10.2 μg/L,
respectively. The developed method was successfully applied to the determination of α-tocopherol in 3 types of commercial
Goji berry products. Average recoveries varied from 85.7% to 106.3%, with RSD in the range of 0.68%–4.62%. In addition,
the DLLME technique had the advantages of simplicity, rapidity and low organic solvent consumption, excellent precision,
high sensitivity and good repeatability.

Key words: dispersive liquid-liquid microextraction, Lycium barbarum, α-tocopherol

中图分类号:

TS207.3

曹江平，刘世巍，解启龙，易宗慧，周继梅，张玉龙. 分散液液微萃取-高效液相色谱法测定枸杞中α-生育酚[J]. 食品科学, doi: 10.7506/spkx1002-6630-201522022.

CAO Jiangping1, LIU Shiwei 1, XIE Qilong2, YI Zonghui1, ZHOU Jimei1, ZHANG Yulong1. Determination of α-Tocopherol in Goji Berries (Lycium barbarum) Using Dispersive Liquid-Liquid Microextraction Combined with High Performance Liquid Chromatography[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201522022.

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分散液液微萃取-高效液相色谱法测定枸杞中α-生育酚

Determination of α-Tocopherol in Goji Berries (Lycium barbarum) Using Dispersive Liquid-Liquid Microextraction Combined with High Performance Liquid Chromatography

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