食品科学

• 营养卫生 • 上一篇    下一篇

蜂王浆对溴代苯诱导小鼠氧化损伤的保护作用

郭亚惠1,刘志勇2,曾志将1,*,陈丽玲2,张 洁2,舒 坤2,周林斌1,潘其忠1   

  1. 1.江西农业大学蜜蜂研究所,江西 南昌 330045;2.江西中医药大学实验动物科技中心,江西 南昌 330006
  • 出版日期:2015-12-15 发布日期:2015-12-24

Protective Effect of Royal Jelly on Bromobenzene-Induced Oxidative Damage in Mice

GUO Yahui1, LIU Zhiyong2, ZENG Zhijiang1,*, CHEN Liling2, ZHANG Jie2, SHU Kun2, ZHOU Linbin1, PAN Qizhong1   

  1. 1. Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China;
    2. Laboratory Animal Science and Technology Center, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China
  • Online:2015-12-15 Published:2015-12-24

摘要:

研究不同取浆时间生产的蜂王浆(royal jelly,RJ)对溴代苯诱导小鼠氧化损伤的保护作用。以免移虫48 h生产的蜂王浆(48 h RJ)和72 h生产的蜂王浆(72 h RJ)为研究材料,灌胃给予昆明小鼠,蜂王浆分别设高剂量组9 g/(kg•d)(48 h RJ-H组、72 h RJ-H组)、中剂量组3 g/(kg•d)(48 h RJ-M组、72 h RJ-M组)和低剂量组1 g/(kg•d)(48 h RJ-L组、72 h RJ-L组),同时设一空白对照组。灌胃剂量为10 mL/kg,空白对照组小鼠则灌胃等量蒸馏水。连续灌胃45 d,然后给各组小鼠灌胃0.3 mg/kg溴代苯油溶液(灌胃剂量为10 mL/kg),22 h后测定小鼠的肝脏质量以及血清和肝脏组织中丙二醛(malondialdehyde,MDA)含量,超氧化物歧化酶(superoxidedismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、总抗氧化能力(total antioxidativecapacity,T-AOC)活性。结果表明:预防性灌胃RJ对溴代苯攻击后小鼠的体质量、肝脏质量和肝脏系数无显著影响(P>0.05);除48 h RJ-L组外,其他组小鼠血清中的MDA含量均显著低于空白对照组(P<0.05),中、高剂量的RJ可显著升高小鼠血清中的SOD和GSH-Px活力,而所有灌胃RJ的小鼠血清T-AOC活力都显著高于空白对照组(P<0.05);灌胃RJ也可显著降低小鼠肝脏组织中的MDA含量和GSH-Px、T-AOC活力(P<0.05),除48 h RJ-L组外,其他组小鼠肝脏组织中SOD活力均显著高于空白对照组(P<0.05)。48 h RJ和72 h RJ对溴代苯诱导小鼠氧化损伤均具有一定的保护作用,但两者存在一定差异。

关键词: 蜂王浆, 取浆时间, 氧化损伤

Abstract:

The aim of this study is to explore the protective effects of royal jelly (RJ) harvested at different time points on
bromobenzene-induced oxidative damage in mice. The 48 h RJ and 72 h RJ were fed to Kunming mice by gavage, each of
which was used to set up a high dose group at 9 g/(kg·d) (48 h RJ-H and 72 h RJ-H), a moderate dose group at 3 g/(kg·d)
(48 h RJ-M and 72 h RJ-M) and a low dose group at 1 g/(kg·d) (48 h RJ-L and 72 h RJ-L), respectively. At the same time, a
control group was set up. Each mouse in the experimental groups was given RJ at a dose of 10 mL/kg, while the control group
mice were given the same amount of distilled water. The mice were fed royal jelly daily by oral gavage for 45 days, and then
given bromobenzene oil at a dose of 0.3 mg/kg (10 mL/kg) by gavage. After 22 h, liver weight and malondialdehyde (MDA)
content, superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity and total antioxidant capacity
(T-AOC) in blood and liver tissue of each mouse were detected. Results showed that preventive administration of RJ had no
significant effect on body weight, liver weight or liver coefficient in bromobenzene-challenged mice (P > 0.05). The level of
serum MDA in the mice from all groups except the 48 h RJ-L group was significantly reduced compared to that in the blank
control group (P < 0.05). Administration of moderate and high-dose RJ caused a significant elevation in the levels of serum
SOD activity and GSH-Px content, and the mice from all groups administered with RJ showed significantly increased levels
of serum T-AOC than the blank control group (P < 0.05). In addition, oral gavage of RJ resulted in a significant reduction in
the levels of MDA, GSH-Px and T-AOC in liver tissue (P < 0.05). The level of SOD activity in liver tissue of the mice from
all groups except the 48 h RJ-L group exhibited a significant increase compared with those from the blank control group
(P < 0.05). Therefore, both 48 h RJ and 72 h RJ have protective effects on bromobenzene-induced oxidative damage in mice.

Key words: royal jelly, harvesting time, oxidative damage

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