食品科学

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虎杖苷对镉致小鼠睾丸氧化应激损伤的保护作用

林 霖1,2,杨国栋1,汪纪仓1,2   

  1. 1.河南科技大学动物科技学院,河南 洛阳 471003;
    2.河南省高等学校环境与畜产品安全重点学科开放实验室,河南 洛阳 471003
  • 出版日期:2015-12-15 发布日期:2015-12-24

Ameliorative Effect of Polydatin on Oxidative Stress Damage Induced by Cadmium in Testis of Mice

LIN Lin1,2, YANG Guodong1, WANG Jicang1,2   

  1. 1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;
    2. Open Laboratory of Key Discipline for Environment and Animal Products Safety of Henan Institute of Higher Learning, Luoyang 471003, China
  • Online:2015-12-15 Published:2015-12-24

摘要:

目的:探讨虎杖苷对镉致小鼠睾丸氧化应激损伤的保护作用。方法:40 只小鼠随机分成5 组:正常对照组,镉组,25、50、100 mg/(kg•d)虎杖苷保护组,于实验第1天,镉组及虎杖苷保护组小鼠一次性腹腔注射2 mg/kg氯化镉(氯化镉质量浓度为0.2 mg/mL),制造小鼠睾丸氧化应激损伤,虎杖苷保护组小鼠同时经口灌胃给予25、50、100 mg/(kg•d)虎杖苷,连续灌胃1 周后处死小鼠,统计小鼠睾丸脏器系数;苏木精-伊红(hematoxylin-eosin,HE)染色,观察小鼠睾丸组织病理学变化;检测虎杖苷对小鼠睾丸超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性,以及丙二醛(malonaldehyde,MDA)和8-羟基脱氧鸟苷(8-hydroxy-2-deoxyguanosine,8-OHdG)含量的影响。结果:与正常对照组比较,镉组小鼠睾丸脏器系数显著降低,睾丸组织细胞变性、坏死,生精细胞明显减少,而虎杖苷保护组小鼠睾丸组织细胞损伤均明显减轻;与正常对照组比较,镉组小鼠睾丸组织SOD及GSH-Px活性均极显著降低,MDA及8-OHdG含量均极显著升高(P<0.01)。与镉组比较,50、100 mg/(kg•d)虎杖苷保护组小鼠的睾丸脏器系数显著或极显著升高(P<0.05或P<0.01),睾丸组织的SOD活性明显升高,差异达到显著或极显著水平(P<0.05或P<0.01);25、50、100 mg/(kg•d)虎杖苷保护组小鼠睾丸组织的GSH-Px活性明显升高,差异达到显著或极显著水平(P<0.05或P<0.01),而MDA及8-OHdG含量均明显降低,差异同样达到显著或极显著水平(P<0.05或P<0.01)。结论:虎杖苷能减轻镉致小鼠睾丸组织细胞脂质过氧化损伤,抑制氧化应激对睾丸细胞DNA的损伤。

关键词: 虎杖苷, 镉, 睾丸损伤, 氧化应激

Abstract:

Objective: To study the protective effect of polydatin on oxidative stress damage induced by cadmium in the
testis of mice. Methods: Forty male mice were randomly divided into five groups including negative control group, cadmium
(Cd) group, and polydatin-protected groups (25, 50 and 100 mg/(kg·d) polydatin). Oxidative stress damage was induced
by 2 mg/kg cadmium chloride on the testis in mice from the Cd group and polydatin-protected groups on the 1st day of the
experiment. On the same day, the mice treated with cadmium except those from the Cd group were subjected to intragastric
administration of polydatin at doses of 25, 50 and 100 mg/(kg·d). The experiment lasted one week. Testis organ coefficient,
the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the contents of MDA and 8-hydroxy-
2-deoxyguanosine (8-OHdG) were examined on testis in each group. Moreover testis tissue morphology was examined by
HE. Results: Testis organ coefficient was decreased, and pathological observations of testis exhibited serious damage with
degeneration, necrosis and decreased number of seminiferous cells in mice from the Cd group, while testis organ coefficient
was enhanced. The damage was slighter in the testis of mice treated with polydatin. Compared with the control group, the
activities of SOD and GSH-Px were decreased remarkably, and the contents of MDA and 8-OHdG were enhanced in the
testis of mice from the Cd group (P < 0.01). However, compared with the Cd group, testis organ coefficient and the activity
of SOD were enhanced significantly in the testis of mice treated with 50 and 100 mg/(kg·d) polydatin (P < 0.05 or P < 0.01),
and the activity of GSH-Px was enhanced significantly in the testis of mice treated with 25, 50 and 100 mg/(kg·d) polydatin
(P < 0.05 or P < 0.01). Moreover, the levels of MDA and 8-OHdG were decreased significantly in the testis of mice treated
with 25, 50 and 100 mg/(kg·d) polydatin (P < 0.05 or P < 0.01). Conclusion: Polydatin has ameliorative effect on testis lipid
peroxidation, and can act as a protector to inhibit DNA damage induced by cadmium.

Key words: polydatin, cadmium, testis damage, oxidative stress

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