食品科学

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响应面试验优化莲蓬壳总黄酮超声提取条件及其抗氧化活性

胡卫成1,王新风1,沈 婷1,王毓宁2,陈 铭1,尤 龙1,纪丽莲1,*,李鹏霞2,*   

  1. 1.淮阴师范学院 江苏省高校区域现代农业与环境保护协同创新中心,淮阴师范学院生命科学学院,江苏省环洪泽湖生态农
    业生物技术重点实验室,江苏 淮安 223300;2.江苏省农业科学院农产品加工研究所,江苏 南京 210014
  • 出版日期:2015-12-25 发布日期:2015-12-24
  • 通讯作者: 纪丽莲,李鹏霞
  • 基金资助:

    江苏省农业科技自主创新资金项目(CX(13)3079)

Optimization of the Extraction Process for Flavonoids from Lotus (Nelumbo nucifera Gaertn.) Receptacle and Their Antioxidant Activities

HU Weicheng1, WANG Xinfeng1, SHEN Ting1, WANG Yuning2, CHEN Ming1, YOU Long1, JI Lilian1,*, LI Pengxia2,*   

  1. 1. Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Jiangsu Collaborative Innovation Center of Regional
    Modern Agriculture & Environmental Protection, Huaiyin Normal University, School of Life Science, Huaiyin Normal University,
    Huai’an 223300, China; 2. Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Online:2015-12-25 Published:2015-12-24
  • Contact: JI Lilian1, LI Pengxia

摘要:

对莲蓬壳总黄酮的超声提取工艺和抗氧化活性进行研究。在单因素试验基础上,采用响应面分析法对超声辅助提取的工艺参数进行优化,得到最优工艺条件为液料比40∶1(mL/g)、乙醇体积分数48%、提取温度60 ℃、提取时间10 min。在此条件下测得莲蓬壳总黄酮提取率为8.32%。抗氧化实验结果表明:莲蓬壳总黄酮具有较强清除1,1-二苯基-2-三硝基苯肼自由基能力和还原力,同时可显著性抑制H2O2诱导的人皮肤纤维细胞氧化应激损伤,是一种极具开发潜力的天然抗氧化剂。

关键词: 响应面分析法, 莲蓬壳, 总黄酮, 超声提取, 自由基

Abstract:

Using Box-Behnken design and response surface methodology, the optimized extraction conditions for maximum
yield (83.2 mg rutin equivalents/g) of total flavonoids from lotus (Nelumbo nucifera Gaertn.) receptacle were determined
as follows: temperature, 60 ℃; ethanol concentration, 48%; ratio of liquid to solid, 40:1 (mL/g); and extraction duration,
10 min. The flavonoids extracted from lotus receptacle (FLR) exerted remarkable scavenging effects on 1,1-diphenyl-2-
picrylhydrazyl (DPPH) free radical and exhibited high reducing power. Moreover, FLR pretreatment effectively elevated cell
viability in H2O2-treated human skin fibroblast cells. The results obtained from in vitro models clearly suggest that FLR, as a
byproduct of the agricultural and food industries, is a potential source of natural antioxidant.

Key words: response surface methodology, lotus receptacle, total flavnoids, ultrasound-assisted extraction, free radicals

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