食品科学

• 成分分析 • 上一篇    下一篇

HPLC-DAD结合交替三线性分解二阶校正法测定荔枝果肉中游离植物甾醇

项雷文1,陈国泰1,翁佳敏1,陈文韬1,汪少芸2,*   

  1. 1.福建师范大学福清分校海洋与生化工程学院,福建 福清 350300;
    2.福州大学生物科学与工程学院,福建 福州 350108
  • 出版日期:2015-12-25 发布日期:2015-12-24
  • 通讯作者: 汪少芸
  • 基金资助:

    福建省省级重点学科“食品科学与工程”建设项目(闽教高[2012]48号);福建省区域科技重大项目(2014N3005)

Determination of Free Phytosterols in Litchi Pulp by HPLC-DAD Coupled with Second-Order Calibration Based on Alternating Trilinear Decomposition

XIANG Leiwen1, CHEN Guotai1, WENG Jiamin1, CHEN Wentao1, WANG Shaoyun2,*   

  1. 1. School of Ocean Science and Biochemistry Engineering, Fuqing Branch of Fujian Normal University, Fuqing 350300, China;
    2. College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China
  • Online:2015-12-25 Published:2015-12-24
  • Contact: WANG Shaoyun

摘要:

为快速测定荔枝果肉中游离植物甾醇含量,建立高效液相色谱-二极管阵列检测器结合基于交替三线性分解算法的二阶校正方法。基本色谱条件:岛津ODS-SP柱(150 mm×4.6 mm,5 μm),流动相体积分数95%乙腈溶液,检测波长范围为190~340 nm,柱温30 ℃,流速1.0 mL/min,进样量20.0 μL。结果表明,‘兰竹’和‘乌叶’荔枝样品中菜油甾醇含量分别为24.1 μg/g和27.4 μg/g,两种荔枝样品豆甾醇含量分别为25.8 μg/g和26.7 μg/g,菜油甾醇和豆甾醇加标回收率分别为(95.25±0.78)%和(96.83±1.01)%。所建立的方法简单、准确、灵敏度高且可靠,可用于检测荔枝果肉中游离植物甾醇含量。

关键词: 高效液相色谱, 二极管阵列检测器, 交替三线性分解, 二阶校正, 植物甾醇

Abstract:

A rapid method to quantify free phytosterols in litchi pulp was established with high performance liquid
chromatography with diode array detection (HPLC-DAD) coupled with second-order calibration method based on
alternating trilinear decomposition (ATLD) algorithm. The chromatographic conditions were simplified by applying secondorder
calibration method. The mobile phase was 95% aqueous acetonitrile (V/V) at a flow rate of 1.0 mL/min. The injection
volume was 20.0 μL. The temperature of inertsil ODS-SP (150 mm × 4.6 mm, 5 μm) column was set at 30 ℃. The detection
wavelengths were 190–340 nm. The average spiked recoveries of campesterol and stigmasterol in litchi pulp were (95.25 ±
0.78)% and (96.83 ± 1.01)%, respectively. The pulp of “Lanzhu” and “Wuye” litchi was detected to contain 24.1 and 27.4 μg/g
campesterol, and 25.8 and 26.7 μg/g stigmasterol, respectively. The newly established method is simple, accurate, highly
sensitive and reliable, and can be used to determine free phytosterols in litchi pulp.

Key words: high performance liquid chromatography (HPLC), diode array detector (DAD), alternating trilinear decomposition (ATLD), second-order calibration, phytosterols

中图分类号: