高效液相色谱-串联质谱同时测定草莓、杨梅中20 种植物生长调节剂残留

doi:10.7506/spkx1002-6630-201524046

食品科学

高效液相色谱-串联质谱同时测定草莓、杨梅中20 种植物生长调节剂残留

虞淼1，吴淑春2,*

1.浙江省农药检定管理所，浙江杭州 310020；2.浙江医学高等专科学校，浙江杭州 310053

出版日期:2015-12-25 发布日期:2015-12-24
通讯作者: 吴淑春
基金资助:
浙江省科技厅分析测试科技计划项目（2013C37069）；浙江省教育厅高等学校访问工程师校企合作项目（FW2013009）

Simultaneous Determination of 20 Plant Growth Regulator Residues in Strawberry and Chinese Bayberry(Myrica rubra) by High Performance Liquid Chromatography-Tandem Mass Spectrometry

YU Miao1, WU Shuchun2,*

1. Institute for the Control of Agrochemicals Zhejiang Province, Hangzhou 310020, China;
2. Zhejiang Medical College, Hangzhou 310053, China

Online:2015-12-25 Published:2015-12-24
Contact: WU Shuchun

摘要/Abstract

摘要：

建立一种高效液相色谱-串联质谱同时测定草莓和杨梅中20 种植物生长调节剂残留的分析方法。样品经含1%乙酸的乙腈溶液提取后，以Phenomenex XB-C18（100 mm×2.1 mm，2.6 μm）为分析色谱柱，甲醇和5 mmol/L乙酸铵-0.1%乙酸缓冲溶液为流动相进行梯度洗脱，基质匹配标准溶液外标法定量。结果表明，20 种植物调节剂在各自的质量浓度范围内线性关系良好（r≥0.990 0），草莓和杨梅中3 个添加量的回收率为70.0%～114.8%，相对标准偏差（n=5）为1.25%～11.1%，检出限在0.14～2.9 μg/kg之间。该方法操作简单、提取效果好，具有良好的灵敏度、回收率和重复性。

关键词: 高效液相色谱-串联质谱, 植物生长调节剂, 草莓, 杨梅, 残留

Abstract:

The present paper describes the development and validation of a method for the simultaneous determination of
20 plant growth regulator residues in strawberry and Chinese bayberry (Myrica rubra) by using high performance liquid
chromatography-tandem mass spectrometry (HPLC-MS/MS). The plant growth regulator residues in 15 g of samples were
extracted with acetonitrile (containing 1% acetic acid) and then analyzed using HPLC-MS/MS. The chromatographic
analysis was carried out on a Phenomenex XB-C18 (100 mm × 2.1 mm, 2.6 μm) column with methanol and 5 mmol/L
ammonium acetate solution containing 0.1% acetic acid as the mobile phases with a gradient program, and the analytes were
quantified by matrix-matched external standard method. The recoveries of 20 plant growth regulators in strawberry and
Chinese bayberry at three spiked levels were 70.0%–114.8%, with relative standard deviation (RSD) between 1.25% and
11.1%. The limits of detection of the method ranged from 0.14 to 2.9 μg/kg. The method is simple, rapid, sensitive, accurate
and repeatable. These figures of merit meet the requirements for the detection of 20 plant growth regulator residues in
strawberry and Chinese bayberry.

Key words: high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), plant growth regulator, strawberry, Chinese bayberry (Myrica rubra), residue

中图分类号:

O657.63

虞淼，吴淑春. 高效液相色谱-串联质谱同时测定草莓、杨梅中20 种植物生长调节剂残留[J]. 食品科学, doi: 10.7506/spkx1002-6630-201524046.

YU Miao1, WU Shuchun2,*. Simultaneous Determination of 20 Plant Growth Regulator Residues in Strawberry and Chinese Bayberry(Myrica rubra) by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201524046.

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高效液相色谱-串联质谱同时测定草莓、杨梅中20 种植物生长调节剂残留

Simultaneous Determination of 20 Plant Growth Regulator Residues in Strawberry and Chinese Bayberry(Myrica rubra) by High Performance Liquid Chromatography-Tandem Mass Spectrometry

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