食品科学

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CdTe量子点作为荧光探针检测皮蛋中微量铜

王蓓蓓1,2,商 欢1,王 琪1,马美湖1,蔡朝霞1,*   

  1. 1.国家蛋品加工技术研发分中心,华中农业大学食品科学技术学院,湖北 武汉 430070;
    2.安徽科技学院食品药品学院,安徽 凤阳 233100
  • 出版日期:2016-01-25 发布日期:2016-01-15
  • 通讯作者: 蔡朝霞
  • 基金资助:

    公益性行业(农业)科研专项(201303084);安徽科技学院校人才引进项目(ZRC2014445)

Ultrasensitive and Rapid Detection of Copper in Preserved Eggs Using CdTe Quantum Dot as a Nano-Fluorescence Probe

WANG Beibei1,2, SHANG Huan1, WANG Qi1, MA Meihu1, CAI Zhaoxia1,*   

  1. 1. National Research and Development Center for Egg Processing, College of Food Science and Technology,
    Huazhong Agricultural University, Wuhan 430070, China;
    2. College of Food and Drug, Anhui Science and Technology University, Fengyang 233100, China
  • Online:2016-01-25 Published:2016-01-15
  • Contact: CAI Zhaoxia

摘要:

基于CdTe量子点作为荧光探针建立了一种定量检测皮蛋中微量铜(Cu2+)的荧光分析法。采用巯基乙酸和1-硫丙三醇作为稳定剂,水相中快速制得荧光性好、稳定性好的水溶性CdTe量子点,并运用紫外-可见分光光度和荧光光谱法研究CdTe量子点的发光特性。实验探究了Cu2+对CdTe量子点荧光猝灭的机理,并根据Cu2+浓度和荧光强度下降量之间的关系,在Cu2+浓度范围为10~200 nmol/L时建立定量检测微量Cu2+的线性方程,相关系数高达0.997,检出限低至0.96 nmol/L。本实验方法已经初步应用于皮蛋样品中Cu2+的检测,结果显示相对标准偏差在1.47%~3.01%之间,回收率为104%~108%,说明了此方法的准确性、可靠性和适用性。

关键词: 量子点, 荧光猝灭, 铜离子, 皮蛋

Abstract:

In this study, highly-fluorescent and water-soluble CdTe quantum dots (QDs) were used as fluorescence probe
to quantitatively detect Cu2+ in preserved egg samples. Using thioglycollic acid (TGA) and thioglycerol (TGOL) as the
stabilizers, the CdTe QDs were synthesized in water phase. The luminescent characteristics of CdTe QDs were studied
using UV-visible spectrophotometry and fluorescence spectroscopy. This experiment investigated the mechanism of the
fluorescence quenching effect of Cu2+ on CdTe QDs and based on the quenching effect, a method for detecting trace Cu2+
using QDs as a probe was established. The results showed the relationship between Cu2+ concentration in the range from 10
to 200 nmol/L and the quenching intensity of CdTe QDs (F) was fairly linear with high correlation coefficient (R2 = 0.997).
The limit of detection was 0.96 nmol/L, and the developed method presented relatively high selectivity and sensitivity. This
method was successfully applied to the analysis of preserved egg samples. The precision, expressed as relative standard
deviation (RSD), was 1.47%–3.01%, and the recovery rate was between 104% and 108%.

Key words: quantum dots, fluorescence quenching, Cu2+, preserved egg

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