食品科学

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拮抗菌Burkholderia contaminans对玫瑰香葡萄采后灰霉病的抗性诱导

范三红1,李 静1,施俊凤2   

  1. 1.山西大学生命科学学院,山西 太原 030006;2.山西省农业科学院农产品贮藏保鲜研究所,山西 太原 030031
  • 出版日期:2016-01-25 发布日期:2016-01-15
  • 基金资助:

    山西省农业科学院博士后基金项目(BSH-2015JJ-003);山西省农业科学院自主创新项目(2015ZZCX-16);
    国家自然科学基金面上项目(31371868)

Induction of Disease Resistance against Botrytis cinerea in Postharvest Muscat Grape by Antagonistic Bacterium Burkholderia contaminans

FAN Sanhong1, LI Jing1, SHI Junfeng2   

  1. 1. College of Life Science, Shanxi University, Taiyuan 030006, China;
    2. Institute of Agricultural Product Storage and Fresh Keeping, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China
  • Online:2016-01-25 Published:2016-01-15

摘要:

以玫瑰香葡萄为实验试材,研究洋葱霍尔德氏菌(Burkholderia contaminans B-1)对玫瑰香葡萄采后灰霉病的抗性诱导机理,对经拮抗菌处理贮藏后果实的腐烂率及其抗性相关物质进行测定。结果表明:拮抗菌B. contaminans B-1可有效降低玫瑰香葡萄采后腐烂率,与对照相比,果梗腐烂率降低69.58%,果肉腐烂指数降低63.93%。拮抗菌处理对果实苯丙烷代谢途径、活性氧代谢途径和其他抗性相关酶类活性均有影响。与对照相比,B. contaminans B-1处理可提高葡萄果实苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、超氧化物歧化酶(SOD)和多酚氧化酶(PPO)活性,促进过氧化氢(H2O2)含量积累,延缓过氧化氢酶(CAT)活性峰值出现的时间。此外,拮抗菌处理也可以诱导果实病程相关蛋白几丁质酶(CHI)和β-1,3葡聚糖酶(GLU)活性的上升。

关键词: 玫瑰香葡萄, 生物防治, 抗性诱导

Abstract:

In this study, the mechanism by which the antagonistic bacterium Burkholderia contaminans B-1 induces
resistance to Botrytis cinerea in postharvest Muscat grape berry was investigated. The results showed that B. contaminans
B-1 reduced significantly the rot rate of postharvest grape berries. Compared with the control, the rot rates of the stem and
fruit treated with B. contaminans were decreased by 69.58% and 63.93%, respectively. B. contaminans B-1 could affect the
phenylpropanoid metabolic pathway, reactive oxygen species metabolic pathway, and the activities of resistance-related
enzymes. Treatment with B. contaminans B-1 enhanced the activities of phenylalanine ammonia-lyase (PAL), peroxidase
(POD), superoxide dismutase (SOD) and polyphenloxidase (PPO). B. contaminans B-1 also increased hydrogen peroxide
(H2O2) content and delayed the peak time of catalase (CAT) activity, and enhanced the activities of resistance-related
enzymes, chitinase (CHI) and β-1,3-glucanase (GLU), in grapes.

Key words: Muscat grape, biological control, induced disease resistance

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