食品科学

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蜂王浆在贮藏过程中5-羟甲基糠醛、10-羟基-2-癸烯酸及精氨酸双糖苷含量的变化

王露露1,2,董 蕊1,范 超1,张 晶2,*   

  1. 1.吉林农业大学中药材学院,吉林 长春 130118;2. 长春科技学院医学院,吉林 长春 130061
  • 出版日期:2016-01-25 发布日期:2016-01-15
  • 通讯作者: 张 晶
  • 基金资助:

    吉林省科技发展计划项目(20120236)

Changes in the Contents of 5-HMF, 10-HDA and AFG in Royal Jelly during Storage

WANG Lulu1,2, DONG Rui1, FAN Chao1, ZHANG Jing2,*   

  1. 1. College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China;
    2. College of Pharmacy, Changchun University of Science and Technology, Changchun 130061, China
  • Online:2016-01-25 Published:2016-01-15
  • Contact: ZHANG Jing

摘要:

将10 个蜂王浆样品贮藏在不同温度(室温、4、-20、-80 ℃)和时间(15、45、65、80 d)条件下,采用高效液相色谱法检测其5-羟甲基糠醛(5-hydroxymethylfurfural,5-HMF)、10-羟基-2-癸烯酸(10-hydroxy-2-decanoicacid,10-HDA)和精氨酸双糖苷(arginyl fructosyl glucose,AFG)含量的变化。结果表明,蜂王浆样品中10-HDA的含量稳定,不随贮藏温度和时间的变化而变化;5-HMF和AFG的含量随贮藏温度升高及时间的延长而增加。因此5-HMF和AFG可作为衡量蜂王浆新鲜程度的指标性成分,而10-HDA不可以作为此标准。但10-HDA是蜂王浆中特有成分,其含量高低会直接影响蜂王浆的功效,故5-HMF、AFG与10-HDA三者结合对蜂王浆的品质进行评价会更加准确。

关键词: 蜂王浆, 5-羟甲基糠醛, 10-羟基-2-癸烯酸, 精氨酸双糖苷, 品质评价

Abstract:

This study examined the effects of different storage temperatures and storage times on the contents of
5-hydroxymethylfurfural (5-HMF), 10-hydroxy-2-decanoic acid (10-HDA) and arginyl fructosyl glucose (AFG) in 10 royal
jelly samples by high performance liquid chromatography (HPLC). The results showed that the content of 10-HDA was
stable and did not change with storage temperature and time. However, the contents of 5-HMF and AFG in royal jelly were
increased with increasing storage temperature and prolonging storage time. Thus, 5-HMF and AFG but not 10-HDA can be
used as indicators to evaluate the freshness of royal jelly. In addition, 10-HDA was the endemic component in royal jelly,
and its content directly influenced the health benefits of royal jelly. Therefore, the combination of 5-HMF, 10-HAD and
AFG enables accurate evaluation of the quality of royal jelly.

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