食品科学

• 基础研究 • 上一篇    下一篇

4 个葡萄品种葡萄籽冷榨油的性质与体外抗氧化活性

郑亚蕾1,刘 叶1,隋银强1,王 华1,2,3,*,李 华1,2,3   

  1. 1.西北农林科技大学葡萄酒学院,陕西 杨凌 712100;2.陕西省葡萄与葡萄酒工程技术研究中心,陕西 杨凌 712100;
    3.合阳葡萄试验示范站,陕西 渭南 715300
  • 出版日期:2016-02-15 发布日期:2016-02-26

Characterization and in Vitro Antioxidant Activity of Cold-Pressed Seed Oils from Four Different Grape Varieties

ZHENG Yalei1, LIU Ye1, SUI Yinqiang1, WANG Hua1,2,3,*, LI Hua1,2,3   

  1. 1. College of Enology, Northwest A&F University, Yangling 712100, China; 2. Shaanxi Engineering Research Center for
    Viti-Viniculture, Yangling 712100, China; 3. Heyang Experimental and Demonstrational Stations for Grape, Weinan 715300, China
  • Online:2016-02-15 Published:2016-02-26

摘要:

目的:评价4 种葡萄籽冷榨油脂肪酸组成、总酚含量及体外抗氧化活性。方法:收集单品种葡萄籽榨油,气相色谱法测定脂肪酸组成,Folin-酚法测定总酚含量,用1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picryhydrazyl,DPPH)法、2,2’-联氮双-(3-乙基苯并噻唑林-6-磺酸)二胺盐(2,2’-azinobis(3-ethylbenzothi azoline-6-sulfonic acid)ammonium salt,ABTS)法、Fenton反应和总抗氧化能力法分析其体外抗氧化能力。结果:4 个品种葡萄籽冷榨油均富含亚油酸(72.77%~77.36%),不饱和脂肪酸含量为88.12%~91.06%;总酚含量为60.77~100.53 μg GAE/g,爱格丽葡萄籽冷榨油总酚含量最高,其次是北冰红、媚丽、赤霞珠葡萄籽冷榨油;4 个品种的葡萄籽冷榨油都有良好的清除DPPH自由基和ABTS+·的能力,且随着葡萄籽冷榨油浓度的增大,清除能力增强,清除DPPH自由基的IC50变化范围为10.19~13.82 mg/mL,清除ABTS+·的IC50变化范围为11.62~19.25 mg/mL;清除羟自由基(·OH)的能力范围为13.18~27.08 U/mL;总抗氧化能力范围为1.08~3.68 U/mL。4 个品种的葡萄籽冷榨油的体外抗氧化能力强弱顺序为爱格丽>北冰红>媚丽>赤霞珠。

关键词: 葡萄籽冷榨油, 多酚, 体外抗氧化活性

Abstract:

Objective: To evaluate the fatty acid composition and total polyphenol contents of cold-pressed seed oils from
four different grape varieties and assay their in vitro antioxidant activity. Methods: The fatty acid composition was measured
by gas chromatography with flame ionization detection (GC-FID). The content of polyphenols was determined using the
Folin-Ciocalteau method and the in vitro antioxidant activity was tested by 1,1-diphenyl-2-picryhydrazyl (DPPH)
radical scavenging, 2,2’-azinobis-3-ethylbenzthi azoline-6-sulfonic acid (ABTS) radical scavenging and Fenton
reaction methods. Results: The most abundant fatty acid in all cold-pressed grape seed oils was linoleic acid (72.77%–
77.36%) and the content of unsaturated fatty acids ranged from 88.12% to 91.06%. Total polyphenols ranged from 60.77
to 100.53 μg GAE/g and the order of total polyphenol contents in grape seed oils from different grape varieties was Ecolly >
Beibinghong > Meili > Carbernet Sauvignon. All clod-pressed grape seed oils had strong scavenging activity against
DPPH, ABTS and hydroxyl free radicals in a dose-dependent manner, with IC50 values ranging from 10.19 to
13.82 mg/mL, from 11.62 to 19.25 mg/mL, and from 13.18 to 27.08 U/mL, respectively. The IC50 value of total
antioxidant capacity ranged from 1.08 to 3.68 U/mL. The order of in vitro antioxidant activity of cold-pressed grape
seed oils was Ecolly > Beibinghong > Meili > Carbernet Sauvignon.

Key words: cold-pressed grape seed oil, polyphenols, in vitro antioxidant activity

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