3 种脂环酸芽孢杆菌分离培养基的性能评价

doi:10.7506/spkx1002-6630-201603032

摘要/Abstract

摘要：

目的：比较3 个厂家的K培养基、酵母粉淀粉葡萄糖（yeast extract starch glucose medium，YSG）培养基和耐酸耐热芽孢菌（Alicyclobacillus）培养基（Bacillus acidoterrestris medium，BAT）对脂环酸芽孢杆菌的检测效果。方法：选用标准菌株、分离株及实际样品对3 个厂家（广东环凯微生物科技有限公司（简称HK，下同）、厂家Ⅰ和厂家Ⅱ）的K培养基、YSG培养基和BAT培养基进行生长菌落数及分离效果的评估。结果：酸土脂环酸芽孢杆菌标准株和脂环酸芽孢杆菌分离株4-2在K、YSG和BAT 3 种培养基上生长的菌落数（3 个厂家培养基上的菌落数平均数）均无显著性差异（P＞0.05）；酸热脂环酸芽孢杆菌标准株和脂环酸芽孢杆菌分离株5-3在K培养基上不生长，在YSG培养基和BAT培养基上生长良好，其菌落平均数统计学上无显著性差异（P＞0.05）；对实际样品中酸土脂环酸芽孢杆菌的分离，3 个厂家的同种培养基分离率和符合率相当；对实际样品中酸热脂环酸芽孢杆菌的分离，在YSG和BAT培养基上，HK和厂家Ⅱ的分离率和符合率相当，优于厂家Ⅰ。结论：K培养基不适合所有脂环芽孢杆菌，但分离酸土脂环酸芽孢杆菌效果很好，YSG培养基和BAT培养基适合所有脂环酸芽孢杆菌。HK和厂家Ⅱ的培养基优于厂家Ⅰ。

关键词: 脂环酸酸芽孢杆菌, 培养基, 性能评价

Abstract:

Objective: To evaluate the efficacy of three media, K agar, yeast extract starch glucose (YSG) medium and Bacillus
acidoterrestris (BAT) agar medium each from three manufacturers (Guangdong huankai, manufacturer II and manufacturer
I), in detecting Alicyclobacillus. Methods: The colony growth of standard strains and the separation of Alicyclobacillus
isolates from real samples were evaluated using the three media. Results: The number of colonies of Alicyclobacillus
acidoterrestris reference culture and Alicyclobacillus isolate 4-2 in K medium, YSG medium and BAT medium did not
significantly differ (P > 0.05). Similarly, there was no significant difference in the number of colonies of A. acidoterrestris
reference culture and Alicyclobacillus isolate 5-3 in YSG medium and BAT medium (P > 0.05), but neither could grow in
K medium. The separation and consistency rates of three media from three manufacturers for isolating A. acidoterrestris
in samples were similar. The separation and consistency rates of YSG medium for isolating A. acidocaldarius in samples
were similar to those of BAT medium from HK and manufacturer II, which were better than those from manufacturer I.
Conclusion: K medium was more effective for isolating A. acidoterrestris, but it was not suitable for isolating all
Alicyclobacillus isolates. YSG medium and BAT medium were suitable for isolating all Alicyclobacillus isolates. The media
from HK and manufacturer II had superior performance than those from manufacturer I.

Key words: Alicyclobacillus, culture media, performance evaluation

中图分类号:

TS201.3

何天文，卢勉飞，蔡芷荷，周梅连，吴清平. 3 种脂环酸芽孢杆菌分离培养基的性能评价[J]. 食品科学, doi: 10.7506/spkx1002-6630-201603032.

HE Tianwen, LU Mianfei, CAI Zhihe, ZHOU Meilian, WU Qingping. Performance Evaluation of Three Media for Alicyclobacillus Isolation[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201603032.

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3 种脂环酸芽孢杆菌分离培养基的性能评价

Performance Evaluation of Three Media for Alicyclobacillus Isolation

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